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An array of 60,000 antibodies for proteome-scale antibody generation and target discovery.

Publication ,  Journal Article
Wang, Z; Li, Y; Hou, B; Pronobis, MI; Wang, M; Wang, Y; Cheng, G; Weng, W; Wang, Y; Tang, Y; Xu, X; Pan, R; Lin, F; Wang, N; Chen, Z ...
Published in: Sci Adv
March 2020

Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic antibody feature. Distinctive combinations of 10,000 to 20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-protein pairs were found for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for membrane proteins and chromatin immunoprecipitation-sequencing mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues identified internalizing tumor antigens for antibody-drug conjugates. By finding high-affinity mAbs at a fraction of current time and cost, PETAL enables proteome-scale antibody generation and target discovery.

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Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

March 2020

Volume

6

Issue

11

Start / End Page

eaax2271

Location

United States

Related Subject Headings

  • U937 Cells
  • THP-1 Cells
  • Proteome
  • Peptides
  • PC-3 Cells
  • Mice
  • MCF-7 Cells
  • K562 Cells
  • Jurkat Cells
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Wang, Z., Li, Y., Hou, B., Pronobis, M. I., Wang, M., Wang, Y., … Meng, X. (2020). An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. Sci Adv, 6(11), eaax2271. https://doi.org/10.1126/sciadv.aax2271
Wang, Zhaohui, Yang Li, Bing Hou, Mira I. Pronobis, Mingqiao Wang, Yuemeng Wang, Guangcun Cheng, et al. “An array of 60,000 antibodies for proteome-scale antibody generation and target discovery.Sci Adv 6, no. 11 (March 2020): eaax2271. https://doi.org/10.1126/sciadv.aax2271.
Wang Z, Li Y, Hou B, Pronobis MI, Wang M, Wang Y, et al. An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. Sci Adv. 2020 Mar;6(11):eaax2271.
Wang, Zhaohui, et al. “An array of 60,000 antibodies for proteome-scale antibody generation and target discovery.Sci Adv, vol. 6, no. 11, Mar. 2020, p. eaax2271. Pubmed, doi:10.1126/sciadv.aax2271.
Wang Z, Li Y, Hou B, Pronobis MI, Wang M, Wang Y, Cheng G, Weng W, Tang Y, Xu X, Pan R, Lin F, Wang N, Chen Z, Wang S, Ma LZ, Huang D, Jiang L, Zeng W, Zhang Y, Du X, Lin Y, Li Z, Xia Q, Geng J, Dai H, Yu Y, Zhao X-D, Yuan Z, Yan J, Nie Q, Zhang X, Wang K, Chen F, Zhang Q, Zhu Y, Zheng S, Poss KD, Tao S-C, Meng X. An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. Sci Adv. 2020 Mar;6(11):eaax2271.

Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

March 2020

Volume

6

Issue

11

Start / End Page

eaax2271

Location

United States

Related Subject Headings

  • U937 Cells
  • THP-1 Cells
  • Proteome
  • Peptides
  • PC-3 Cells
  • Mice
  • MCF-7 Cells
  • K562 Cells
  • Jurkat Cells
  • Humans