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Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b.

Publication ,  Journal Article
Weber, C; Zhou, Y; Lee, JG; Looger, LL; Qian, G; Ge, C; Capel, B
Published in: Science
April 17, 2020

In many reptiles, including the red-eared slider turtle Trachemys scripta elegans (T. scripta), sex is determined by ambient temperature during embryogenesis. We previously showed that the epigenetic regulator Kdm6b is elevated at the male-producing temperature and essential to activate the male pathway. In this work, we established a causal link between temperature and transcriptional regulation of Kdm6b We show that signal transducer and activator of transcription 3 (STAT3) is phosphorylated at the warmer, female-producing temperature, binds the Kdm6b locus, and represses Kdm6b transcription, blocking the male pathway. Influx of Ca2+, a mediator of STAT3 phosphorylation, is elevated at the female temperature and acts as a temperature-sensitive regulator of STAT3 activation.

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Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 17, 2020

Volume

368

Issue

6488

Start / End Page

303 / 306

Location

United States

Related Subject Headings

  • Turtles
  • Transcription, Genetic
  • Temperature
  • Sex Determination Processes
  • STAT3 Transcription Factor
  • Phosphorylation
  • Male
  • Jumonji Domain-Containing Histone Demethylases
  • General Science & Technology
  • Gene Expression Regulation, Developmental
 

Citation

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Weber, C., Zhou, Y., Lee, J. G., Looger, L. L., Qian, G., Ge, C., & Capel, B. (2020). Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b. Science, 368(6488), 303–306. https://doi.org/10.1126/science.aaz4165
Weber, Ceri, Yingjie Zhou, Jong Gwan Lee, Loren L. Looger, Guoying Qian, Chutian Ge, and Blanche Capel. “Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b.Science 368, no. 6488 (April 17, 2020): 303–6. https://doi.org/10.1126/science.aaz4165.
Weber C, Zhou Y, Lee JG, Looger LL, Qian G, Ge C, et al. Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b. Science. 2020 Apr 17;368(6488):303–6.
Weber, Ceri, et al. “Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b.Science, vol. 368, no. 6488, Apr. 2020, pp. 303–06. Pubmed, doi:10.1126/science.aaz4165.
Weber C, Zhou Y, Lee JG, Looger LL, Qian G, Ge C, Capel B. Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b. Science. 2020 Apr 17;368(6488):303–306.
Journal cover image

Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 17, 2020

Volume

368

Issue

6488

Start / End Page

303 / 306

Location

United States

Related Subject Headings

  • Turtles
  • Transcription, Genetic
  • Temperature
  • Sex Determination Processes
  • STAT3 Transcription Factor
  • Phosphorylation
  • Male
  • Jumonji Domain-Containing Histone Demethylases
  • General Science & Technology
  • Gene Expression Regulation, Developmental