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Click by Click Microporous Annealed Particle (MAP) Scaffolds.

Publication ,  Journal Article
Darling, NJ; Xi, W; Sideris, E; Anderson, AR; Pong, C; Carmichael, ST; Segura, T
Published in: Advanced healthcare materials
May 2020

Macroporous scaffolds are being increasingly used in regenerative medicine and tissue repair. While the recently developed microporous annealed particle (MAP) scaffolds have overcome issues with injectability and in situ hydrogel formation, limitations with respect to tunability to be able to manipulate hydrogel strength and rigidity for broad applications still exist. To address these key issues, here hydrogel microparticles (HMPs) of hyaluronic acid (HA) are synthesized using the thiol-norbornene click reaction and then HMPs are subsequently annealed into a porous scaffold using the tetrazine-norbornene click reaction. This assembly method allows for straightforward tuning of bulk scaffold rigidity by varying the tetrazine to norbornene ratio, with increasing tetrazine resulting in increasing scaffold storage modulus, Young's modulus, and maximum stress. These changes are independent of void fraction. Further incorporation of human dermal fibroblasts throughout the porous scaffold reveals the biocompatibility of this annealing strategy as well as differences in proliferation and cell-occupied volume. Finally, injection of porous HA-Tet MAP scaffolds into an ischemic stroke model shows this chemistry is biocompatible in vivo with reduced levels of inflammation and astrogliosis as previously demonstrated for other crosslinking chemistries.

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Published In

Advanced healthcare materials

DOI

EISSN

2192-2659

ISSN

2192-2640

Publication Date

May 2020

Volume

9

Issue

10

Start / End Page

e1901391

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Regenerative Medicine
  • Porosity
  • Hydrogels
  • Hyaluronic Acid
  • Humans
  • Fibroblasts
  • Biocompatible Materials
  • 4003 Biomedical engineering
 

Citation

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Darling, N. J., Xi, W., Sideris, E., Anderson, A. R., Pong, C., Carmichael, S. T., & Segura, T. (2020). Click by Click Microporous Annealed Particle (MAP) Scaffolds. Advanced Healthcare Materials, 9(10), e1901391. https://doi.org/10.1002/adhm.201901391
Darling, Nicole J., Weixian Xi, Elias Sideris, Alexa R. Anderson, Cassie Pong, S Thomas Carmichael, and Tatiana Segura. “Click by Click Microporous Annealed Particle (MAP) Scaffolds.Advanced Healthcare Materials 9, no. 10 (May 2020): e1901391. https://doi.org/10.1002/adhm.201901391.
Darling NJ, Xi W, Sideris E, Anderson AR, Pong C, Carmichael ST, et al. Click by Click Microporous Annealed Particle (MAP) Scaffolds. Advanced healthcare materials. 2020 May;9(10):e1901391.
Darling, Nicole J., et al. “Click by Click Microporous Annealed Particle (MAP) Scaffolds.Advanced Healthcare Materials, vol. 9, no. 10, May 2020, p. e1901391. Epmc, doi:10.1002/adhm.201901391.
Darling NJ, Xi W, Sideris E, Anderson AR, Pong C, Carmichael ST, Segura T. Click by Click Microporous Annealed Particle (MAP) Scaffolds. Advanced healthcare materials. 2020 May;9(10):e1901391.
Journal cover image

Published In

Advanced healthcare materials

DOI

EISSN

2192-2659

ISSN

2192-2640

Publication Date

May 2020

Volume

9

Issue

10

Start / End Page

e1901391

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Regenerative Medicine
  • Porosity
  • Hydrogels
  • Hyaluronic Acid
  • Humans
  • Fibroblasts
  • Biocompatible Materials
  • 4003 Biomedical engineering