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Granulocytes act as a niche for Mycobacterium tuberculosis growth.

Publication ,  Journal Article
Lovewell, RR; Baer, CE; Mishra, BB; Smith, CM; Sassetti, CM
Published in: Mucosal Immunol
January 2021

Granulocyte recruitment to the pulmonary compartment is a hallmark of progressive tuberculosis (TB). This process is well-documented to promote immunopathology, but can also enhance the replication of the pathogen. Both the specific granulocytes responsible for increasing mycobacterial burden and the underlying mechanisms remain obscure. We report that the known immunomodulatory effects of these cells, such as suppression of protective T-cell responses, play a limited role in altering host control of mycobacterial replication in susceptible mice. Instead, we find that the adaptive immune response preferentially restricts the burden of bacteria within monocytes and macrophages compared to granulocytes. Specifically, mycobacteria within inflammatory lesions are preferentially found within long-lived granulocytes that express intermediate levels of the Ly6G marker and low levels of antimicrobial genes. These cells progressively accumulate in the lung and correlate with bacterial load and disease severity, and the ablation of Ly6G-expressing cells lowers mycobacterial burden. These observations suggest a model in which dysregulated granulocytic influx promotes disease by creating a permissive intracellular niche for mycobacterial growth and persistence.

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Published In

Mucosal Immunol

DOI

EISSN

1935-3456

Publication Date

January 2021

Volume

14

Issue

1

Start / End Page

229 / 241

Location

United States

Related Subject Headings

  • Tuberculosis
  • Severity of Illness Index
  • Mycobacterium tuberculosis
  • Mice, Knockout
  • Mice
  • Lymphocyte Depletion
  • Inflammation Mediators
  • Immunophenotyping
  • Immunology
  • Host-Pathogen Interactions
 

Citation

APA
Chicago
ICMJE
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Lovewell, R. R., Baer, C. E., Mishra, B. B., Smith, C. M., & Sassetti, C. M. (2021). Granulocytes act as a niche for Mycobacterium tuberculosis growth. Mucosal Immunol, 14(1), 229–241. https://doi.org/10.1038/s41385-020-0300-z
Lovewell, Rustin R., Christina E. Baer, Bibhuti B. Mishra, Clare M. Smith, and Christopher M. Sassetti. “Granulocytes act as a niche for Mycobacterium tuberculosis growth.Mucosal Immunol 14, no. 1 (January 2021): 229–41. https://doi.org/10.1038/s41385-020-0300-z.
Lovewell RR, Baer CE, Mishra BB, Smith CM, Sassetti CM. Granulocytes act as a niche for Mycobacterium tuberculosis growth. Mucosal Immunol. 2021 Jan;14(1):229–41.
Lovewell, Rustin R., et al. “Granulocytes act as a niche for Mycobacterium tuberculosis growth.Mucosal Immunol, vol. 14, no. 1, Jan. 2021, pp. 229–41. Pubmed, doi:10.1038/s41385-020-0300-z.
Lovewell RR, Baer CE, Mishra BB, Smith CM, Sassetti CM. Granulocytes act as a niche for Mycobacterium tuberculosis growth. Mucosal Immunol. 2021 Jan;14(1):229–241.

Published In

Mucosal Immunol

DOI

EISSN

1935-3456

Publication Date

January 2021

Volume

14

Issue

1

Start / End Page

229 / 241

Location

United States

Related Subject Headings

  • Tuberculosis
  • Severity of Illness Index
  • Mycobacterium tuberculosis
  • Mice, Knockout
  • Mice
  • Lymphocyte Depletion
  • Inflammation Mediators
  • Immunophenotyping
  • Immunology
  • Host-Pathogen Interactions