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Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration.

Publication ,  Journal Article
Huang, R; Vi, L; Zong, X; Baht, GS
Published in: FASEB J
October 2020

Inflammaging is associated with poor tissue regeneration observed in advanced age. Specifically, protracted inflammation after acute injury has been associated with decreased bone fracture healing and increased rates of nonunion in elderly patients. Here, we investigated the efficacy of using Maresin 1 (MaR1), an omega-3 fatty acid-derived pro-resolving agent, to resolve inflammation after tibial fracture injury and subsequently improving aged bone healing. Aged (24-month-old mice) underwent tibial fracture surgery and were either treated with vehicle or MaR1 3 days after injury. Fracture calluses were harvested 7 days, 14 days, 21 days, and 28 days after injury to investigate inflammatory response, cartilage development, bone deposition, and mechanical integrity, respectively. Healing bones from MaR1-treated mice displayed decreased cartilage formation and increased bone deposition which resulted in increased structural stiffness and increased force to fracture in the later stages of repair. In the early stages, MaR1 treatment decreased the number of pro-inflammatory macrophages within the fracture callus and decreased the level of inflammatory biomarkers in circulation. In tissue culture models, MaR1 treatment of bone marrow-derived macrophages from aged mice protected cells form a pro-inflammatory phenotype and induced an anti-inflammatory fate. Furthermore, the secretome of MaR1-treated bone marrow-derived macrophages was identified as osteoinductive, enhancing osteoblast differentiation of bone marrow stromal cells. Our findings here identify resolution of inflammation, and MaR1 itself, to be a point of intervention to improve aged bone healing.

Duke Scholars

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

October 2020

Volume

34

Issue

10

Start / End Page

13521 / 13532

Location

United States

Related Subject Headings

  • Tibial Fractures
  • Mice
  • Mesenchymal Stem Cells
  • Male
  • Macrophages
  • Inflammation
  • Fracture Healing
  • Female
  • Docosahexaenoic Acids
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Huang, R., Vi, L., Zong, X., & Baht, G. S. (2020). Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration. FASEB J, 34(10), 13521–13532. https://doi.org/10.1096/fj.202001145R
Huang, Rong, Linda Vi, Xiaohua Zong, and Gurpreet S. Baht. “Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration.FASEB J 34, no. 10 (October 2020): 13521–32. https://doi.org/10.1096/fj.202001145R.
Huang R, Vi L, Zong X, Baht GS. Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration. FASEB J. 2020 Oct;34(10):13521–32.
Huang, Rong, et al. “Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration.FASEB J, vol. 34, no. 10, Oct. 2020, pp. 13521–32. Pubmed, doi:10.1096/fj.202001145R.
Huang R, Vi L, Zong X, Baht GS. Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration. FASEB J. 2020 Oct;34(10):13521–13532.

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

October 2020

Volume

34

Issue

10

Start / End Page

13521 / 13532

Location

United States

Related Subject Headings

  • Tibial Fractures
  • Mice
  • Mesenchymal Stem Cells
  • Male
  • Macrophages
  • Inflammation
  • Fracture Healing
  • Female
  • Docosahexaenoic Acids
  • Cells, Cultured