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Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice.

Publication ,  Journal Article
Periera-Simon, S; Xia, X; Catanuto, P; Coronado, R; Kurtzberg, J; Bellio, M; Lee, Y-S; Khan, A; Smith, R; Elliot, SJ; Glassberg, MK
Published in: Respirology
February 2021

BACKGROUND AND OBJECTIVE: IPF is a fatal and debilitating lung disorder increasing in incidence worldwide. To date, two approved treatments only slow disease progression, have multiple side effects and do not provide a cure. MSC have promising therapeutic potential as a cell-based therapy for many lung disorders based on the anti-fibrotic properties of the MSC. METHODS: Critical questions remain surrounding the optimal source, timing and efficacy of cell-based therapies. The present study examines the most effective sources of MSC. Human MSC were derived from adipose, WJ, chorionic membrane (CSC) and chorionic villi (CVC). MSC were injected into the ageing mouse model of BLM-induced lung fibrosis. RESULTS: All sources decreased Aschroft and hydroxyproline levels when injected into BLM-treated mice at day 10 with the exception of CSC cells that did not change hydroxyproline levels. There were also decreases in mRNA expression of αv -integrin and TNFα in all sources except CSC. Only ASC- and WJ-derived cells reduced AKT and MMP-2 activation, while Cav-1 was increased by ASC treatment as previously reported. BLM-induced miR dysregulation of miR-29 and miR-199 was restored only by ASC treatment. CONCLUSION: Our data suggest that sources of MSC may differ in the pathway(s) involved in repair.

Duke Scholars

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Published In

Respirology

DOI

EISSN

1440-1843

Publication Date

February 2021

Volume

26

Issue

2

Start / End Page

161 / 170

Location

Australia

Related Subject Headings

  • Transplantation, Homologous
  • Respiratory System
  • RNA, Messenger
  • Pulmonary Fibrosis
  • Proto-Oncogene Proteins c-akt
  • MicroRNAs
  • Mice, Inbred C57BL
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Matrix Metalloproteinase 2
 

Citation

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Periera-Simon, S., Xia, X., Catanuto, P., Coronado, R., Kurtzberg, J., Bellio, M., … Glassberg, M. K. (2021). Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice. Respirology, 26(2), 161–170. https://doi.org/10.1111/resp.13928
Periera-Simon, Simone, Xiaomei Xia, Paola Catanuto, Ramon Coronado, Joanne Kurtzberg, Michael Bellio, Yee-Shuan Lee, et al. “Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice.Respirology 26, no. 2 (February 2021): 161–70. https://doi.org/10.1111/resp.13928.
Periera-Simon S, Xia X, Catanuto P, Coronado R, Kurtzberg J, Bellio M, et al. Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice. Respirology. 2021 Feb;26(2):161–70.
Periera-Simon, Simone, et al. “Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice.Respirology, vol. 26, no. 2, Feb. 2021, pp. 161–70. Pubmed, doi:10.1111/resp.13928.
Periera-Simon S, Xia X, Catanuto P, Coronado R, Kurtzberg J, Bellio M, Lee Y-S, Khan A, Smith R, Elliot SJ, Glassberg MK. Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice. Respirology. 2021 Feb;26(2):161–170.
Journal cover image

Published In

Respirology

DOI

EISSN

1440-1843

Publication Date

February 2021

Volume

26

Issue

2

Start / End Page

161 / 170

Location

Australia

Related Subject Headings

  • Transplantation, Homologous
  • Respiratory System
  • RNA, Messenger
  • Pulmonary Fibrosis
  • Proto-Oncogene Proteins c-akt
  • MicroRNAs
  • Mice, Inbred C57BL
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Matrix Metalloproteinase 2