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Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay.

Publication ,  Journal Article
Levin, AE; Williamson, PC; Erwin, JL; Cyrus, S; Bloch, EM; Shaz, BH; Kessler, D; Telford, SR; Krause, PJ; Wormser, GP; Ni, X; Wang, H ...
Published in: Transfusion
September 2014

BACKGROUND: Transfusion-transmitted babesiosis caused by Babesia microti has emerged as a significant risk to the US blood supply. This study estimated the prevalence of B. microti antibodies in blood donors using an investigational enzyme immunoassay (EIA). STUDY DESIGN AND METHODS: A peptide-based EIA that detects both immunoglobulin (Ig)G and IgM antibodies to B. microti was developed and validated. Donor samples randomly selected from areas defined as high-risk endemic, lower-risk endemic, and nonendemic for B. microti were deidentified and tested using the investigational EIA. Samples that were EIA repeat reactive were further tested by B. microti immunofluorescent assay (IFA), polymerase chain reaction (PCR) on red blood cell lysates, and peripheral blood smear examination. A random subset of 1272 samples from high-risk endemic areas was tested by IFA, PCR, and peripheral blood smear in parallel with EIA. RESULTS: Among 15,000 donations tested with the investigational B. microti EIA, EIA repeat-reactive rates were 1.08% (54/5000) in a high-risk endemic area, 0.74% (37/5000) in a lower-risk area, and 0.40% (20/5000) in a nonendemic area. After application of a revised cutoff, these values were reduced to 0.92%, (46/5000), 0.54% (27/5000), and 0.16% (8/5000). Overall concordance between EIA and IFA among donor samples was 99.34%. One seropositive sample was positive by PCR. CONCLUSION: The seroprevalence of B. microti in blood donors in a high-risk area measured by an investigational EIA was approximately 1%. The EIA shows promise as an efficient high-throughput blood donor screening assay for B. microti.

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Published In

Transfusion

DOI

EISSN

1537-2995

Publication Date

September 2014

Volume

54

Issue

9

Start / End Page

2237 / 2244

Location

United States

Related Subject Headings

  • Transfusion Reaction
  • Seroepidemiologic Studies
  • Immunoenzyme Techniques
  • Humans
  • Cardiovascular System & Hematology
  • Blood Donors
  • Babesiosis
  • Babesia microti
  • Antibodies, Protozoan
  • 3204 Immunology
 

Citation

APA
Chicago
ICMJE
MLA
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Levin, A. E., Williamson, P. C., Erwin, J. L., Cyrus, S., Bloch, E. M., Shaz, B. H., … Busch, M. P. (2014). Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay. Transfusion, 54(9), 2237–2244. https://doi.org/10.1111/trf.12763
Levin, Andrew E., Phillip C. Williamson, James L. Erwin, Sherri Cyrus, Evan M. Bloch, Beth H. Shaz, Debra Kessler, et al. “Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay.Transfusion 54, no. 9 (September 2014): 2237–44. https://doi.org/10.1111/trf.12763.
Levin AE, Williamson PC, Erwin JL, Cyrus S, Bloch EM, Shaz BH, et al. Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay. Transfusion. 2014 Sep;54(9):2237–44.
Levin, Andrew E., et al. “Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay.Transfusion, vol. 54, no. 9, Sept. 2014, pp. 2237–44. Pubmed, doi:10.1111/trf.12763.
Levin AE, Williamson PC, Erwin JL, Cyrus S, Bloch EM, Shaz BH, Kessler D, Telford SR, Krause PJ, Wormser GP, Ni X, Wang H, Krueger NX, Caglioti S, Busch MP. Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay. Transfusion. 2014 Sep;54(9):2237–2244.
Journal cover image

Published In

Transfusion

DOI

EISSN

1537-2995

Publication Date

September 2014

Volume

54

Issue

9

Start / End Page

2237 / 2244

Location

United States

Related Subject Headings

  • Transfusion Reaction
  • Seroepidemiologic Studies
  • Immunoenzyme Techniques
  • Humans
  • Cardiovascular System & Hematology
  • Blood Donors
  • Babesiosis
  • Babesia microti
  • Antibodies, Protozoan
  • 3204 Immunology