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A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium.

Publication ,  Journal Article
Miao, EA; Miller, SI
Published in: Proc Natl Acad Sci U S A
June 20, 2000

Type III secretion systems (TTSS) are important virulence factors that Gram-negative bacteria use to translocate proteins into the cytoplasm of eukaryotic host cells. Salmonellae encode two virulence-associated TTSS. The Salmonella pathogenicity island 1 (SPI1)-encoded TTSS is active on contact with host cells, whereas the Salmonella pathogenicity island 2 (SPI2)-encoded TTSS is expressed after phagocytosis of bacteria by host cells. Previously, no consensus signal sequence for translocation has been identified among TTSS effector proteins. In this work, seven proteins, termed Salmonella-translocated effectors (STE), are described that contain conserved amino acid sequences that direct translocation by TTSS in Salmonella typhimurium. STE that are coordinately regulated with SPI2 gene expression contain translocation signals that are recognized by the SPI2 but not by the SPI1 TTSS. STE that are constitutively expressed contain signals that direct translocation through both SPI1 and SPI2 TTSS. Of the seven STE examined, SspH1 and SspH2 have been previously shown to be translocated and involved in virulence; SlrP and SifA were identified as virulence factors, but were not previously known to be associated with TTSS; and SseI, SseJ, and SifB were previously unidentified. Three STE genes (sspH1, sspH2, and sseI) are located within temperate bacteriophages, suggesting a common mechanism for the dissemination of more recently evolved STE.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 20, 2000

Volume

97

Issue

13

Start / End Page

7539 / 7544

Location

United States

Related Subject Headings

  • Virulence
  • Sequence Analysis, Protein
  • Sequence Alignment
  • Salmonella typhimurium
  • Open Reading Frames
  • Molecular Sequence Data
  • Humans
  • Genes, Bacterial
  • Cell Line
  • Bacterial Proteins
 

Citation

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Miao, E. A., & Miller, S. I. (2000). A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A, 97(13), 7539–7544. https://doi.org/10.1073/pnas.97.13.7539
Miao, E. A., and S. I. Miller. “A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium.Proc Natl Acad Sci U S A 97, no. 13 (June 20, 2000): 7539–44. https://doi.org/10.1073/pnas.97.13.7539.
Miao EA, Miller SI. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7539–44.
Miao, E. A., and S. I. Miller. “A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium.Proc Natl Acad Sci U S A, vol. 97, no. 13, June 2000, pp. 7539–44. Pubmed, doi:10.1073/pnas.97.13.7539.
Miao EA, Miller SI. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7539–7544.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 20, 2000

Volume

97

Issue

13

Start / End Page

7539 / 7544

Location

United States

Related Subject Headings

  • Virulence
  • Sequence Analysis, Protein
  • Sequence Alignment
  • Salmonella typhimurium
  • Open Reading Frames
  • Molecular Sequence Data
  • Humans
  • Genes, Bacterial
  • Cell Line
  • Bacterial Proteins