Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Pseudomonas aeruginosa activates caspase 1 through Ipaf.

Publication ,  Journal Article
Miao, EA; Ernst, RK; Dors, M; Mao, DP; Aderem, A
Published in: Proc Natl Acad Sci U S A
February 19, 2008

The innate immune system encodes cytosolic Nod-like receptors (NLRs), several of which activate caspase 1 processing and IL-1beta and IL-18 secretion. Macrophages respond to Salmonella typhimurium infection by activating caspase 1 through the NLR Ipaf. This activation is mediated by cytosolic flagellin through the activity of the virulence-associated type III secretion system (T3SS). We demonstrate here that Pseudomonas aeruginosa activates caspase 1 and induces IL-1beta secretion in infected macrophages. While live, virulent P. aeruginosa activate IL-1beta secretion through caspase 1 and Ipaf, strains that have mutations in the T3SS or in flagellin did not. Ipaf-dependent caspase 1 activation could be recapitulated by delivering P. aeruginosa flagellin to the macrophage cytosol. We examined the role of Naip5 in P. aeruginosa-induced caspase 1 activation by using A/J (Naip5-deficient) compared with C57BL/6 and BALB/c (Naip5-sufficient) macrophages and observed that A/J macrophages secrete IL-1beta in response to P. aeruginosa, S. typhimurium, and Listeria monocytogenes infection, as well as in response to cytosolic flagellin, but at slightly reduced levels. Thus, Ipaf-dependent detection of cytosolic flagellin is a conserved mechanism by which macrophages detect the presence of pathogens that use T3SS.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

February 19, 2008

Volume

105

Issue

7

Start / End Page

2562 / 2567

Location

United States

Related Subject Headings

  • Pseudomonas aeruginosa
  • Protein Transport
  • Neuronal Apoptosis-Inhibitory Protein
  • Macrophages
  • Interleukin-1beta
  • Flagellin
  • Enzyme Activation
  • Cytosol
  • Cells, Cultured
  • Caspase 1
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Miao, E. A., Ernst, R. K., Dors, M., Mao, D. P., & Aderem, A. (2008). Pseudomonas aeruginosa activates caspase 1 through Ipaf. Proc Natl Acad Sci U S A, 105(7), 2562–2567. https://doi.org/10.1073/pnas.0712183105
Miao, Edward A., Robert K. Ernst, Monica Dors, Dat P. Mao, and Alan Aderem. “Pseudomonas aeruginosa activates caspase 1 through Ipaf.Proc Natl Acad Sci U S A 105, no. 7 (February 19, 2008): 2562–67. https://doi.org/10.1073/pnas.0712183105.
Miao EA, Ernst RK, Dors M, Mao DP, Aderem A. Pseudomonas aeruginosa activates caspase 1 through Ipaf. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2562–7.
Miao, Edward A., et al. “Pseudomonas aeruginosa activates caspase 1 through Ipaf.Proc Natl Acad Sci U S A, vol. 105, no. 7, Feb. 2008, pp. 2562–67. Pubmed, doi:10.1073/pnas.0712183105.
Miao EA, Ernst RK, Dors M, Mao DP, Aderem A. Pseudomonas aeruginosa activates caspase 1 through Ipaf. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2562–2567.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

February 19, 2008

Volume

105

Issue

7

Start / End Page

2562 / 2567

Location

United States

Related Subject Headings

  • Pseudomonas aeruginosa
  • Protein Transport
  • Neuronal Apoptosis-Inhibitory Protein
  • Macrophages
  • Interleukin-1beta
  • Flagellin
  • Enzyme Activation
  • Cytosol
  • Cells, Cultured
  • Caspase 1