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Disease-Specific Expression of Conjunctiva Associated Lymphoid Tissue (CALT) in Mouse Models of Dry Eye Disease and Ocular Allergy.

Publication ,  Journal Article
Steven, P; Schwab, S; Kiesewetter, A; Saban, DR; Stern, ME; Gehlsen, U
Published in: International journal of molecular sciences
October 2020

Conjunctiva-associated tissue (CALT) is assumed to play a crucial role in the immune system of the ocular surface. Its function in several ocular surface diseases (OSD) is still not fully understood. This study investigates the function of CALT in mouse models of dry-eye disease and ocular allergy. Since antigen-presentation is the central similarity in the pathologies, this study focuses on antigen-presentation in CALT Morphology and the expression of CALT, which was investigated in mice after induction of dry-eye, ocular allergy, topical antigen-stimulation, and after local depletion of phagocytic cells. Antigen uptake was investigated after the application of fluorescent ovalbumin (OVA). OSD influences the appearance and morphology of CALT in a disease-dependent manner. Ocular allergy leads to an increase and dry-eye disease to a decrease in number and size of CALT. The development of CALT is dependent on the presence of APCs. Professional APCs are present in CALT, and soluble antigen is transported into the follicle. CALT appearance is disease-specific and indicative of differing functions. Although the specific involvement of CALT in OSD needs further study, the existence of functional APCS and antigen-uptake supports the hypothesis that CALT is an immunological key player at the ocular surface.

Duke Scholars

Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

October 2020

Volume

21

Issue

20

Start / End Page

E7514

Related Subject Headings

  • Mice
  • Lymphoid Tissue
  • Immunohistochemistry
  • Hypersensitivity
  • Gene Expression Regulation
  • Fluorescent Antibody Technique
  • Female
  • Dry Eye Syndromes
  • Disease Models, Animal
  • Conjunctiva
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Steven, P., Schwab, S., Kiesewetter, A., Saban, D. R., Stern, M. E., & Gehlsen, U. (2020). Disease-Specific Expression of Conjunctiva Associated Lymphoid Tissue (CALT) in Mouse Models of Dry Eye Disease and Ocular Allergy. International Journal of Molecular Sciences, 21(20), E7514. https://doi.org/10.3390/ijms21207514
Steven, Philipp, Sebastian Schwab, Anne Kiesewetter, Daniel R. Saban, Michael E. Stern, and Uta Gehlsen. “Disease-Specific Expression of Conjunctiva Associated Lymphoid Tissue (CALT) in Mouse Models of Dry Eye Disease and Ocular Allergy.International Journal of Molecular Sciences 21, no. 20 (October 2020): E7514. https://doi.org/10.3390/ijms21207514.
Steven P, Schwab S, Kiesewetter A, Saban DR, Stern ME, Gehlsen U. Disease-Specific Expression of Conjunctiva Associated Lymphoid Tissue (CALT) in Mouse Models of Dry Eye Disease and Ocular Allergy. International journal of molecular sciences. 2020 Oct;21(20):E7514.
Steven, Philipp, et al. “Disease-Specific Expression of Conjunctiva Associated Lymphoid Tissue (CALT) in Mouse Models of Dry Eye Disease and Ocular Allergy.International Journal of Molecular Sciences, vol. 21, no. 20, Oct. 2020, p. E7514. Epmc, doi:10.3390/ijms21207514.
Steven P, Schwab S, Kiesewetter A, Saban DR, Stern ME, Gehlsen U. Disease-Specific Expression of Conjunctiva Associated Lymphoid Tissue (CALT) in Mouse Models of Dry Eye Disease and Ocular Allergy. International journal of molecular sciences. 2020 Oct;21(20):E7514.

Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

October 2020

Volume

21

Issue

20

Start / End Page

E7514

Related Subject Headings

  • Mice
  • Lymphoid Tissue
  • Immunohistochemistry
  • Hypersensitivity
  • Gene Expression Regulation
  • Fluorescent Antibody Technique
  • Female
  • Dry Eye Syndromes
  • Disease Models, Animal
  • Conjunctiva