Demineralized Murine Skeletal Histology.
Publication
, Journal Article
Mirando, AJ; Hilton, MJ
Published in: Methods Mol Biol
2021
Cartilage and bone are specialized skeletal tissues composed of unique extracellular matrices. Bone, in particular, has a highly calcified or mineralized matrix that makes microtomy and standard histological studies very challenging. Therefore, methods to appropriately fix and decalcify mineralized skeletal tissues have been developed to allow for paraffin processing and standard microtomy. In this chapter, we will illustrate methods for tissue grossing, fixation, decalcification, paraffin processing, embedding, sectioning, and routine histological staining of demineralized murine skeletal tissues. We will also discuss methods for decalcified frozen sectioning of skeletal tissues with and without the use of a tape-transfer system.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2230
Start / End Page
283 / 302
Location
United States
Related Subject Headings
- Tissue Fixation
- Staining and Labeling
- Paraffin Embedding
- Microtomy
- Mice
- Frozen Sections
- Developmental Biology
- Decalcification Technique
- Cartilage
- Bone and Bones
Citation
APA
Chicago
ICMJE
MLA
NLM
Mirando, A. J., & Hilton, M. J. (2021). Demineralized Murine Skeletal Histology. Methods Mol Biol, 2230, 283–302. https://doi.org/10.1007/978-1-0716-1028-2_16
Mirando, Anthony J., and Matthew J. Hilton. “Demineralized Murine Skeletal Histology.” Methods Mol Biol 2230 (2021): 283–302. https://doi.org/10.1007/978-1-0716-1028-2_16.
Mirando AJ, Hilton MJ. Demineralized Murine Skeletal Histology. Methods Mol Biol. 2021;2230:283–302.
Mirando, Anthony J., and Matthew J. Hilton. “Demineralized Murine Skeletal Histology.” Methods Mol Biol, vol. 2230, 2021, pp. 283–302. Pubmed, doi:10.1007/978-1-0716-1028-2_16.
Mirando AJ, Hilton MJ. Demineralized Murine Skeletal Histology. Methods Mol Biol. 2021;2230:283–302.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2230
Start / End Page
283 / 302
Location
United States
Related Subject Headings
- Tissue Fixation
- Staining and Labeling
- Paraffin Embedding
- Microtomy
- Mice
- Frozen Sections
- Developmental Biology
- Decalcification Technique
- Cartilage
- Bone and Bones