Whole Mount In Situ Hybridization in Murine Tissues.
Publication
, Journal Article
Sharma, D; Hilton, MJ; Karner, CM
Published in: Methods Mol Biol
2021
Whole mount in situ hybridization is a sensitive method used to characterize the spatial and temporal expression of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying gene expression during embryonic development. Here, we describe a procedure for digoxigenin labeled in situ hybridization on whole embryos.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2230
Start / End Page
367 / 376
Location
United States
Related Subject Headings
- RNA Probes
- Pregnancy
- Mice
- In Situ Hybridization
- Gene Expression Regulation, Developmental
- Female
- Embryonic Development
- Embryo, Mammalian
- Digoxigenin
- Developmental Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Sharma, D., Hilton, M. J., & Karner, C. M. (2021). Whole Mount In Situ Hybridization in Murine Tissues. Methods Mol Biol, 2230, 367–376. https://doi.org/10.1007/978-1-0716-1028-2_22
Sharma, Deepika, Matthew J. Hilton, and Courtney M. Karner. “Whole Mount In Situ Hybridization in Murine Tissues.” Methods Mol Biol 2230 (2021): 367–76. https://doi.org/10.1007/978-1-0716-1028-2_22.
Sharma D, Hilton MJ, Karner CM. Whole Mount In Situ Hybridization in Murine Tissues. Methods Mol Biol. 2021;2230:367–76.
Sharma, Deepika, et al. “Whole Mount In Situ Hybridization in Murine Tissues.” Methods Mol Biol, vol. 2230, 2021, pp. 367–76. Pubmed, doi:10.1007/978-1-0716-1028-2_22.
Sharma D, Hilton MJ, Karner CM. Whole Mount In Situ Hybridization in Murine Tissues. Methods Mol Biol. 2021;2230:367–376.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2230
Start / End Page
367 / 376
Location
United States
Related Subject Headings
- RNA Probes
- Pregnancy
- Mice
- In Situ Hybridization
- Gene Expression Regulation, Developmental
- Female
- Embryonic Development
- Embryo, Mammalian
- Digoxigenin
- Developmental Biology