Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage.
Publication
, Journal Article
Liao, Y; Long, JT; Gallo, CJR; Mirando, AJ; Hilton, MJ
Published in: Methods Mol Biol
2021
Primary chondrocyte isolation and culture is a useful tool to characterize how cellular perturbations impact chondrocyte behavior and mineralization in vitro. This protocol conveys methods for isolating and culturing primary chondrocytes from costal and growth plate cartilage. Following gross dissection of the neonatal murine anterior rib cage or long bone growth plate cartilage, chondrocytes are isolated via enzymatic digestion and plated at high density. Genetic perturbation of plated primary murine chondrocytes using viral infection of Cre recombinase to excise floxed alleles and/or overexpress genes of interest are also described.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2230
Start / End Page
415 / 423
Location
United States
Related Subject Headings
- Primary Cell Culture
- Mice
- Growth Plate
- Developmental Biology
- Chondrocytes
- Cell Separation
- Cartilage
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Liao, Y., Long, J. T., Gallo, C. J. R., Mirando, A. J., & Hilton, M. J. (2021). Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage. Methods Mol Biol, 2230, 415–423. https://doi.org/10.1007/978-1-0716-1028-2_25
Liao, Yihan, Jason T. Long, Christopher J. R. Gallo, Anthony J. Mirando, and Matthew J. Hilton. “Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage.” Methods Mol Biol 2230 (2021): 415–23. https://doi.org/10.1007/978-1-0716-1028-2_25.
Liao Y, Long JT, Gallo CJR, Mirando AJ, Hilton MJ. Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage. Methods Mol Biol. 2021;2230:415–23.
Liao, Yihan, et al. “Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage.” Methods Mol Biol, vol. 2230, 2021, pp. 415–23. Pubmed, doi:10.1007/978-1-0716-1028-2_25.
Liao Y, Long JT, Gallo CJR, Mirando AJ, Hilton MJ. Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage. Methods Mol Biol. 2021;2230:415–423.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2230
Start / End Page
415 / 423
Location
United States
Related Subject Headings
- Primary Cell Culture
- Mice
- Growth Plate
- Developmental Biology
- Chondrocytes
- Cell Separation
- Cartilage
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology