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RNA editing generates tissue-specific sodium channels with distinct gating properties.

Publication ,  Journal Article
Song, W; Liu, Z; Tan, J; Nomura, Y; Dong, K
Published in: The Journal of biological chemistry
July 2004

Sodium channels play an essential role in generating the action potential in eukaryotic cells, and their transcripts, especially those in insects, undergo extensive A-to-I RNA editing. The functional consequences of RNA editing of sodium channel transcripts, however, have yet to be determined. We characterized 20 splice variants of the German cockroach sodium channel gene BgNa(v). Functional analysis revealed that these variants exhibited a broad range of voltage-dependent activation and inactivation. Further analysis of two variants, BgNa(v)1-1 and BgNa(v)1-2, which activate at more depolarizing membrane potentials than other variants, showed that RNA editing events were responsible for variant-specific gating properties. Two U-to-C editing sites identified in BgNa(v)1-1 resulted in a Leu to Pro change in segment 1 of domain III (IIIS1) and a Val to Ala change in IVS4. The Leu to Pro change shifted both the voltage dependence of activation and steady-state inactivation in the depolarizing direction. Two A-to-I editing events in BgNa(v)1-2 resulted in a Lys to Arg change in IS2 and an Ile to Met change in IVS3. The Lys to Arg change shifted the voltage dependence of activation in the depolarizing direction. Moreover, these RNA editing events occurred in a tissue-specific and development-specific manner. Our findings provide direct evidence that RNA editing is an important mechanism generating tissue-/cell type-specific functional variants of sodium channels.

Duke Scholars

Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

July 2004

Volume

279

Issue

31

Start / End Page

32554 / 32561

Related Subject Headings

  • Xenopus
  • Sodium Channels
  • Sodium
  • RNA, Messenger
  • RNA Editing
  • Oocytes
  • Oligonucleotides
  • Nerve Tissue Proteins
  • NAV1.2 Voltage-Gated Sodium Channel
  • NAV1.1 Voltage-Gated Sodium Channel
 

Citation

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Song, W., Liu, Z., Tan, J., Nomura, Y., & Dong, K. (2004). RNA editing generates tissue-specific sodium channels with distinct gating properties. The Journal of Biological Chemistry, 279(31), 32554–32561. https://doi.org/10.1074/jbc.m402392200
Song, Weizhong, Zhiqi Liu, Jianguo Tan, Yoshiko Nomura, and Ke Dong. “RNA editing generates tissue-specific sodium channels with distinct gating properties.The Journal of Biological Chemistry 279, no. 31 (July 2004): 32554–61. https://doi.org/10.1074/jbc.m402392200.
Song W, Liu Z, Tan J, Nomura Y, Dong K. RNA editing generates tissue-specific sodium channels with distinct gating properties. The Journal of biological chemistry. 2004 Jul;279(31):32554–61.
Song, Weizhong, et al. “RNA editing generates tissue-specific sodium channels with distinct gating properties.The Journal of Biological Chemistry, vol. 279, no. 31, July 2004, pp. 32554–61. Epmc, doi:10.1074/jbc.m402392200.
Song W, Liu Z, Tan J, Nomura Y, Dong K. RNA editing generates tissue-specific sodium channels with distinct gating properties. The Journal of biological chemistry. 2004 Jul;279(31):32554–32561.

Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

July 2004

Volume

279

Issue

31

Start / End Page

32554 / 32561

Related Subject Headings

  • Xenopus
  • Sodium Channels
  • Sodium
  • RNA, Messenger
  • RNA Editing
  • Oocytes
  • Oligonucleotides
  • Nerve Tissue Proteins
  • NAV1.2 Voltage-Gated Sodium Channel
  • NAV1.1 Voltage-Gated Sodium Channel