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Effects of intravesical prostaglandin E2 on bladder function are preserved in capsaicin-desensitized rats.

Publication ,  Journal Article
Hokanson, JA; Langdale, CL; Milliken, PH; Sridhar, A; Grill, WM
Published in: American journal of physiology. Renal physiology
February 2021

Prostaglandin E2 (PGE2) instilled into the bladder generates symptoms of urinary urgency in healthy women and reduces bladder capacity and urethral pressure in both humans and female rats. Systemic capsaicin desensitization, which causes degeneration of C-fibers, prevented PGE2-mediated reductions in bladder capacity, suggesting that PGE2 acts as an irritant (Maggi CA, Giuliani S, Conte B, Furio M, Santicioli P, Meli P, Gragnani L, Meli A. Eur J Pharmacol 145: 105-112, 1988). In the present study, we instilled PGE2 in female rats after capsaicin desensitization but without the hypogastric nerve transection that was conducted in the Maggi et al. study. One week after capsaicin injection (125 mg/kg sc), rats underwent cystometric and urethral perfusion testing under urethane anesthesia with saline and 100 µM PGE2. Similar to naïve rats, capsaicin-desensitized rats exhibited a reduction in bladder capacity from 1.23 ± 0.08 mL to 0.70 ± 0.10 mL (P = 0.002, n = 9), a reduction in urethral perfusion pressure from 19.3 ± 2.1 cmH2O to 10.9 ± 1.2 cmH2O (P = 0.004, n = 9), and a reduction in bladder compliance from 0.13 ± 0.020 mL/cmH2O to 0.090 ± 0.014 mL/cmH2O (P = 0.011, n = 9). Thus, changes in bladder function following the instillation of PGE2 were not dependent on capsaicin-sensitive pathways. Further, these results suggest that urethral relaxation/weakness and/or increased detrusor pressure as a result of decreased compliance may contribute to urinary urgency and highlight potential targets for new therapies for overactive bladder.

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Published In

American journal of physiology. Renal physiology

DOI

EISSN

1522-1466

ISSN

1931-857X

Publication Date

February 2021

Volume

320

Issue

2

Start / End Page

F212 / F223

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Sensory System Agents
  • Rats, Wistar
  • Rats
  • Oxytocics
  • Female
  • Dinoprostone
  • Capsaicin
  • Animals
 

Citation

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Hokanson, J. A., Langdale, C. L., Milliken, P. H., Sridhar, A., & Grill, W. M. (2021). Effects of intravesical prostaglandin E2 on bladder function are preserved in capsaicin-desensitized rats. American Journal of Physiology. Renal Physiology, 320(2), F212–F223. https://doi.org/10.1152/ajprenal.00302.2020
Hokanson, James A., Christopher L. Langdale, Philip H. Milliken, Arun Sridhar, and Warren M. Grill. “Effects of intravesical prostaglandin E2 on bladder function are preserved in capsaicin-desensitized rats.American Journal of Physiology. Renal Physiology 320, no. 2 (February 2021): F212–23. https://doi.org/10.1152/ajprenal.00302.2020.
Hokanson JA, Langdale CL, Milliken PH, Sridhar A, Grill WM. Effects of intravesical prostaglandin E2 on bladder function are preserved in capsaicin-desensitized rats. American journal of physiology Renal physiology. 2021 Feb;320(2):F212–23.
Hokanson, James A., et al. “Effects of intravesical prostaglandin E2 on bladder function are preserved in capsaicin-desensitized rats.American Journal of Physiology. Renal Physiology, vol. 320, no. 2, Feb. 2021, pp. F212–23. Epmc, doi:10.1152/ajprenal.00302.2020.
Hokanson JA, Langdale CL, Milliken PH, Sridhar A, Grill WM. Effects of intravesical prostaglandin E2 on bladder function are preserved in capsaicin-desensitized rats. American journal of physiology Renal physiology. 2021 Feb;320(2):F212–F223.

Published In

American journal of physiology. Renal physiology

DOI

EISSN

1522-1466

ISSN

1931-857X

Publication Date

February 2021

Volume

320

Issue

2

Start / End Page

F212 / F223

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Sensory System Agents
  • Rats, Wistar
  • Rats
  • Oxytocics
  • Female
  • Dinoprostone
  • Capsaicin
  • Animals