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Colonic Epithelial-Derived Selenoprotein P Is the Source for Antioxidant-Mediated Protection in Colitis-Associated Cancer.

Publication ,  Journal Article
Short, SP; Pilat, JM; Barrett, CW; Reddy, VK; Haberman, Y; Hendren, JR; Marsh, BJ; Keating, CE; Motley, AK; Hill, KE; Zemper, AE; Shi, C ...
Published in: Gastroenterology
April 2021

BACKGROUND & AIMS: Patients with inflammatory bowel disease (IBD) demonstrate nutritional selenium deficiencies and are at greater risk of developing colon cancer. Previously, we determined that global reduction of the secreted antioxidant selenium-containing protein, selenoprotein P (SELENOP), substantially increased tumor development in an experimental colitis-associated cancer (CAC) model. We next sought to delineate tissue-specific contributions of SELENOP to intestinal inflammatory carcinogenesis and define clinical context. METHODS: Selenop floxed mice crossed with Cre driver lines to delete Selenop from the liver, myeloid lineages, or intestinal epithelium were placed on an azoxymethane/dextran sodium sulfate experimental CAC protocol. SELENOP loss was assessed in human ulcerative colitis (UC) organoids, and expression was queried in human and adult UC samples. RESULTS: Although large sources of SELENOP, both liver- and myeloid-specific Selenop deletion failed to modify azoxymethane/dextran sodium sulfate-mediated tumorigenesis. Instead, epithelial-specific deletion increased CAC tumorigenesis, likely due to elevated oxidative stress with a resulting increase in genomic instability and augmented tumor initiation. SELENOP was down-regulated in UC colon biopsies and levels were inversely correlated with endoscopic disease severity and tissue S100A8 (calprotectin) gene expression. CONCLUSIONS: Although global selenium status is typically assessed by measuring liver-derived plasma SELENOP levels, our results indicate that the peripheral SELENOP pool is dispensable for CAC. Colonic epithelial SELENOP is the main contributor to local antioxidant capabilities. Thus, colonic SELENOP is the most informative means to assess selenium levels and activity in IBD patients and may serve as a novel biomarker for UC disease severity and identify patients most predisposed to CAC development.

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Published In

Gastroenterology

DOI

EISSN

1528-0012

Publication Date

April 2021

Volume

160

Issue

5

Start / End Page

1694 / 1708.e3

Location

United States

Related Subject Headings

  • Selenoprotein P
  • Oxidative Stress
  • Myeloid Cells
  • Mice, Knockout
  • Mice
  • Male
  • Liver
  • Intestinal Mucosa
  • Humans
  • Genomic Instability
 

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Short, S. P., Pilat, J. M., Barrett, C. W., Reddy, V. K., Haberman, Y., Hendren, J. R., … Williams, C. S. (2021). Colonic Epithelial-Derived Selenoprotein P Is the Source for Antioxidant-Mediated Protection in Colitis-Associated Cancer. Gastroenterology, 160(5), 1694-1708.e3. https://doi.org/10.1053/j.gastro.2020.12.059
Short, Sarah P., Jennifer M. Pilat, Caitlyn W. Barrett, Vishruth K. Reddy, Yael Haberman, Jared R. Hendren, Benjamin J. Marsh, et al. “Colonic Epithelial-Derived Selenoprotein P Is the Source for Antioxidant-Mediated Protection in Colitis-Associated Cancer.Gastroenterology 160, no. 5 (April 2021): 1694-1708.e3. https://doi.org/10.1053/j.gastro.2020.12.059.
Short SP, Pilat JM, Barrett CW, Reddy VK, Haberman Y, Hendren JR, et al. Colonic Epithelial-Derived Selenoprotein P Is the Source for Antioxidant-Mediated Protection in Colitis-Associated Cancer. Gastroenterology. 2021 Apr;160(5):1694-1708.e3.
Short, Sarah P., et al. “Colonic Epithelial-Derived Selenoprotein P Is the Source for Antioxidant-Mediated Protection in Colitis-Associated Cancer.Gastroenterology, vol. 160, no. 5, Apr. 2021, pp. 1694-1708.e3. Pubmed, doi:10.1053/j.gastro.2020.12.059.
Short SP, Pilat JM, Barrett CW, Reddy VK, Haberman Y, Hendren JR, Marsh BJ, Keating CE, Motley AK, Hill KE, Zemper AE, Washington MK, Shi C, Chen X, Wilson KT, Hyams JS, Denson LA, Burk RF, Rosen MJ, Williams CS. Colonic Epithelial-Derived Selenoprotein P Is the Source for Antioxidant-Mediated Protection in Colitis-Associated Cancer. Gastroenterology. 2021 Apr;160(5):1694-1708.e3.
Journal cover image

Published In

Gastroenterology

DOI

EISSN

1528-0012

Publication Date

April 2021

Volume

160

Issue

5

Start / End Page

1694 / 1708.e3

Location

United States

Related Subject Headings

  • Selenoprotein P
  • Oxidative Stress
  • Myeloid Cells
  • Mice, Knockout
  • Mice
  • Male
  • Liver
  • Intestinal Mucosa
  • Humans
  • Genomic Instability