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Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella).

Publication ,  Journal Article
Li, B-J; Wang, K-K; Chen, D-P; Yan, Y; Cai, X-L; Chen, H-M; Dong, K; Lin, F; Xu, H-H
Published in: Insect science
December 2021

The phenylpyrazole insecticide fipronil blocks resistance to dieldrin (RDL) γ-aminobutyric acid (GABA) receptors in insects, thereby impairing inhibitory neurotransmission. Some insect species, such as the diamondback moth (Plutella xylostella), possess more than one Rdl gene. The involvement of multiple Rdls in fipronil toxicity and resistance remains largely unknown. In this study, we investigated the roles of two Rdl genes, PxRdl1 and PxRdl2, in P. xylostella fipronil action. In Xenopus oocytes, PxRDL2 receptors were 40 times less sensitive to fipronil than PxRDL1. PxRDL2 receptors were also less sensitive to GABA compared with PxRDL1. Knockout of the fipronil-sensitive PxRdl1 reduced the fipronil potency 10-fold, whereas knockout of the fipronil-resistant PxRdl2 enhanced the fipronil potency 4.4-fold. Furthermore, in two fipronil-resistant diamondback moth field populations, PxRdl2 expression was elevated 3.7- and 4.1-fold compared with a susceptible strain, whereas PxRdl1 expression was comparable among the resistant and susceptible strains. Collectively, our results indicate antagonistic effects of PxRDL1 and PxRDL2 on fipronil action in vivo and suggest that enhanced expression of fipronil-resistant PxRdl2 is potentially a new mechanism of fipronil resistance in insects.

Duke Scholars

Published In

Insect science

DOI

EISSN

1744-7917

ISSN

1672-9609

Publication Date

December 2021

Volume

28

Issue

6

Start / End Page

1721 / 1733

Related Subject Headings

  • Receptors, GABA
  • Pyrazoles
  • Moths
  • Insecticides
  • Insecticide Resistance
  • Entomology
  • Animals
  • 0608 Zoology
  • 0501 Ecological Applications
 

Citation

APA
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ICMJE
MLA
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Li, B.-J., Wang, K.-K., Chen, D.-P., Yan, Y., Cai, X.-L., Chen, H.-M., … Xu, H.-H. (2021). Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella). Insect Science, 28(6), 1721–1733. https://doi.org/10.1111/1744-7917.12892
Li, Ben-Jie, Kun-Kun Wang, Dong-Ping Chen, Ying Yan, Xu-Ling Cai, Hui-Min Chen, Ke Dong, Fei Lin, and Han-Hong Xu. “Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella).Insect Science 28, no. 6 (December 2021): 1721–33. https://doi.org/10.1111/1744-7917.12892.
Li B-J, Wang K-K, Chen D-P, Yan Y, Cai X-L, Chen H-M, et al. Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella). Insect science. 2021 Dec;28(6):1721–33.
Li, Ben-Jie, et al. “Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella).Insect Science, vol. 28, no. 6, Dec. 2021, pp. 1721–33. Epmc, doi:10.1111/1744-7917.12892.
Li B-J, Wang K-K, Chen D-P, Yan Y, Cai X-L, Chen H-M, Dong K, Lin F, Xu H-H. Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella). Insect science. 2021 Dec;28(6):1721–1733.
Journal cover image

Published In

Insect science

DOI

EISSN

1744-7917

ISSN

1672-9609

Publication Date

December 2021

Volume

28

Issue

6

Start / End Page

1721 / 1733

Related Subject Headings

  • Receptors, GABA
  • Pyrazoles
  • Moths
  • Insecticides
  • Insecticide Resistance
  • Entomology
  • Animals
  • 0608 Zoology
  • 0501 Ecological Applications