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A standard calculation methodology for human doubly labeled water studies.

Publication ,  Journal Article
Speakman, JR; Yamada, Y; Sagayama, H; Berman, ESF; Ainslie, PN; Andersen, LF; Anderson, LJ; Arab, L; Baddou, I; Bedu-Addo, K; Blaak, EE; Hu, S ...
Published in: Cell Rep Med
February 16, 2021
Published version (DOI) Link to item

The doubly labeled water (DLW) method measures total energy expenditure (TEE) in free-living subjects. Several equations are used to convert isotopic data into TEE. Using the International Atomic Energy Agency (IAEA) DLW database (5,756 measurements of adults and children), we show considerable variability is introduced by different equations. The estimated rCO2 is sensitive to the dilution space ratio (DSR) of the two isotopes. Based on performance in validation studies, we propose a new equation based on a new estimate of the mean DSR. The DSR is lower at low body masses (<10 kg). Using data for 1,021 babies and infants, we show that the DSR varies non-linearly with body mass between 0 and 10 kg. Using this relationship to predict DSR from weight provides an equation for rCO2 over this size range that agrees well with indirect calorimetry (average difference 0.64%; SD = 12.2%). We propose adoption of these equations in future studies.

Duke Scholars

William Erle Kraus
Author William Erle Kraus Medicine, Cardiology
Herman Pontzer
Author Herman Pontzer Evolutionary Anthropology
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Published In

Cell Rep Med

DOI

10.1016/j.xcrm.2021.100203

EISSN

2666-3791

Publication Date

February 16, 2021

Volume

2

Issue

2

Start / End Page

100203

Location

United States

Related Subject Headings

  • Water
  • Oxygen Isotopes
  • Humans
  • Energy Metabolism
  • Deuterium
  • Calorimetry, Indirect
  • Body Composition
  • 32 Biomedical and clinical sciences
 

Citation

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Speakman, J. R., Yamada, Y., Sagayama, H., Berman, E. S. F., Ainslie, P. N., Andersen, L. F., … IAEA DLW database group. (2021). A standard calculation methodology for human doubly labeled water studies. Cell Rep Med, 2(2), 100203. https://doi.org/10.1016/j.xcrm.2021.100203
Speakman, John R., Yosuke Yamada, Hiroyuki Sagayama, Elena S. F. Berman, Philip N. Ainslie, Lene F. Andersen, Liam J. Anderson, et al. “A standard calculation methodology for human doubly labeled water studies.” Cell Rep Med 2, no. 2 (February 16, 2021): 100203. https://doi.org/10.1016/j.xcrm.2021.100203.
Speakman JR, Yamada Y, Sagayama H, Berman ESF, Ainslie PN, Andersen LF, et al. A standard calculation methodology for human doubly labeled water studies. Cell Rep Med. 2021 Feb 16;2(2):100203.
Speakman, John R., et al. “A standard calculation methodology for human doubly labeled water studies.” Cell Rep Med, vol. 2, no. 2, Feb. 2021, p. 100203. Pubmed, doi:10.1016/j.xcrm.2021.100203.
Speakman JR, Yamada Y, Sagayama H, Berman ESF, Ainslie PN, Andersen LF, Anderson LJ, Arab L, Baddou I, Bedu-Addo K, Blaak EE, Blanc S, Bonomi AG, Bouten CVC, Bovet P, Buchowski MS, Butte NF, Camps SGJA, Close GL, Cooper JA, Creasy SA, Das SK, Cooper R, Dugas LR, Ebbeling CB, Ekelund U, Entringer S, Forrester T, Fudge BW, Goris AH, Gurven M, Hambly C, El Hamdouchi A, Hoos MB, Hu S, Joonas N, Joosen AM, Katzmarzyk P, Kempen KP, Kimura M, Kraus WE, Kushner RF, Lambert EV, Leonard WR, Lessan N, Ludwig DS, Martin CK, Medin AC, Meijer EP, Morehen JC, Morton JP, Neuhouser ML, Nicklas TA, Ojiambo RM, Pietiläinen KH, Pitsiladis YP, Plange-Rhule J, Plasqui G, Prentice RL, Rabinovich RA, Racette SB, Raichlen DA, Ravussin E, Reynolds RM, Roberts SB, Schuit AJ, Sjödin AM, Stice E, Urlacher SS, Valenti G, Van Etten LM, Van Mil EA, Wells JCK, Wilson G, Wood BM, Yanovski J, Yoshida T, Zhang X, Murphy-Alford AJ, Loechl CU, Melanson EL, Luke AH, Pontzer H, Rood J, Schoeller DA, Westerterp KR, Wong WW, IAEA DLW database group. A standard calculation methodology for human doubly labeled water studies. Cell Rep Med. 2021 Feb 16;2(2):100203.

Published In

Cell Rep Med

DOI

10.1016/j.xcrm.2021.100203

EISSN

2666-3791

Publication Date

February 16, 2021

Volume

2

Issue

2

Start / End Page

100203

Location

United States

Related Subject Headings

  • Water
  • Oxygen Isotopes
  • Humans
  • Energy Metabolism
  • Deuterium
  • Calorimetry, Indirect
  • Body Composition
  • 32 Biomedical and clinical sciences