Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.
Publication
, Journal Article
Abutaleb, NO; Truskey, GA
Published in: STAR protocols
June 2021
Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).
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Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
June 2021
Volume
2
Issue
2
Start / End Page
100394
Related Subject Headings
- Stress, Physiological
- Stress, Mechanical
- Induced Pluripotent Stem Cells
- Humans
- Endothelial Cells
- Cells, Cultured
- Cell Differentiation
- Cell Culture Techniques
Citation
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Chicago
ICMJE
MLA
NLM
Abutaleb, N. O., & Truskey, G. A. (2021). Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress. STAR Protocols, 2(2), 100394. https://doi.org/10.1016/j.xpro.2021.100394
Abutaleb, Nadia O., and George A. Truskey. “Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.” STAR Protocols 2, no. 2 (June 2021): 100394. https://doi.org/10.1016/j.xpro.2021.100394.
Abutaleb NO, Truskey GA. Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress. STAR protocols. 2021 Jun;2(2):100394.
Abutaleb, Nadia O., and George A. Truskey. “Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.” STAR Protocols, vol. 2, no. 2, June 2021, p. 100394. Epmc, doi:10.1016/j.xpro.2021.100394.
Abutaleb NO, Truskey GA. Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress. STAR protocols. 2021 Jun;2(2):100394.
Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
June 2021
Volume
2
Issue
2
Start / End Page
100394
Related Subject Headings
- Stress, Physiological
- Stress, Mechanical
- Induced Pluripotent Stem Cells
- Humans
- Endothelial Cells
- Cells, Cultured
- Cell Differentiation
- Cell Culture Techniques