Skip to main content
Journal cover image

Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models.

Publication ,  Journal Article
Vincent, A; Thauvin, M; Quévrain, E; Mathieu, E; Layani, S; Seksik, P; Batinic-Haberle, I; Vriz, S; Policar, C; Delsuc, N
Published in: J Inorg Biochem
June 2021

Oxidative stress that results from an imbalance between the concentrations of reactive species (RS) and antioxidant defenses is associated with many pathologies. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase are among the key enzymes that maintain the low nanomolar physiological concentrations of superoxide and hydrogen peroxide. The increase in the levels of these species and their progeny could have deleterious effects. In this context, chemists have developed SOD and CAT mimics to supplement them when cells are overwhelmed with oxidative stress. However, the beneficial activity of such molecules in cells depends not only on their intrinsic catalytic activities but also on their stability in biological context, their cell penetration and their cellular localization. We have employed cellular assays to characterize several compounds that possess SOD and CAT activities and have been frequently used in cellular and animal models. We used cellular assays that address SOD and CAT activities of the compounds. Finally, we determined the effect of compounds on the suppression of the inflammation in HT29-MD2 cells challenged by lipopolysaccharide. When the assay requires penetration inside cells, the SOD mimics Mn(III) meso-tetrakis(N-(2'-n-butoxyethyl)pyridinium-2-yl)porphyrin (MnTnBuOE-2-PyP5+) and Mn(II) dichloro[(4aR,13aR,17aR,21aR)-1,2,3,4,4a,5,6,12,13,13a,14,15,16,17,17a,18,19,20,21,21a-eicosahydro-11,7-nitrilo-7Hdibenzo[b,h] [1,4, 7,10] tetraazacycloheptadecine-κN5,κN13,κN18,κN21,κN22] (Imisopasem manganese, M40403, CG4419) were found efficacious at 10 μM, while Mn(II) chloro N-(phenolato)-N,N'-bis[2-(N-methyl-imidazolyl)methyl]-ethane-1,2-diamine (Mn1) requires an incubation at 100 μM. This study thus demonstrates that MnTnBuOE-2-PyP5+, M40403 and Mn1 were efficacious in suppressing inflammatory response in HT29-MD2 cells and such action appears to be related to their ability to enter the cells and modulate reactive oxygen species (ROS) levels.

Duke Scholars

Published In

J Inorg Biochem

DOI

EISSN

1873-3344

Publication Date

June 2021

Volume

219

Start / End Page

111431

Location

United States

Related Subject Headings

  • Superoxides
  • Superoxide Dismutase
  • Reactive Oxygen Species
  • Porphyrins
  • Oxidative Stress
  • Oxidation-Reduction
  • Organometallic Compounds
  • Molecular Mimicry
  • Metalloporphyrins
  • Manganese
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Vincent, A., Thauvin, M., Quévrain, E., Mathieu, E., Layani, S., Seksik, P., … Delsuc, N. (2021). Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models. J Inorg Biochem, 219, 111431. https://doi.org/10.1016/j.jinorgbio.2021.111431
Vincent, Amandine, Marion Thauvin, Elodie Quévrain, Emilie Mathieu, Sarah Layani, Philippe Seksik, Ines Batinic-Haberle, Sophie Vriz, Clotilde Policar, and Nicolas Delsuc. “Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models.J Inorg Biochem 219 (June 2021): 111431. https://doi.org/10.1016/j.jinorgbio.2021.111431.
Vincent A, Thauvin M, Quévrain E, Mathieu E, Layani S, Seksik P, et al. Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models. J Inorg Biochem. 2021 Jun;219:111431.
Vincent, Amandine, et al. “Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models.J Inorg Biochem, vol. 219, June 2021, p. 111431. Pubmed, doi:10.1016/j.jinorgbio.2021.111431.
Vincent A, Thauvin M, Quévrain E, Mathieu E, Layani S, Seksik P, Batinic-Haberle I, Vriz S, Policar C, Delsuc N. Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models. J Inorg Biochem. 2021 Jun;219:111431.
Journal cover image

Published In

J Inorg Biochem

DOI

EISSN

1873-3344

Publication Date

June 2021

Volume

219

Start / End Page

111431

Location

United States

Related Subject Headings

  • Superoxides
  • Superoxide Dismutase
  • Reactive Oxygen Species
  • Porphyrins
  • Oxidative Stress
  • Oxidation-Reduction
  • Organometallic Compounds
  • Molecular Mimicry
  • Metalloporphyrins
  • Manganese