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Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences.

Publication ,  Journal Article
Clark, SL; Chan, R; Zhao, M; Xie, LY; Copeland, WE; Aberg, KA; van den Oord, EJCG
Published in: J Am Acad Child Adolesc Psychiatry
December 2021

OBJECTIVE: The impact of adolescent cannabis use is a pressing public health question owing to the high rates of use and links to negative outcomes. This study considered the association between problematic adolescent cannabis use and methylation. METHOD: Using an enrichment-based sequencing approach, a methylome-wide association study (MWAS) was performed of problematic adolescent cannabis use in 703 adolescent samples from the Great Smoky Mountain Study. Using epigenomic deconvolution, MWASs were performed for the main cell types in blood: granulocytes, T cells, B cells, and monocytes. Enrichment testing was conducted to establish overlap between cannabis-associated methylation differences and variants associated with negative mental health effects of adolescent cannabis use. RESULTS: Whole-blood analyses identified 45 significant CpGs, and cell type-specific analyses yielded 32 additional CpGs not identified in the whole-blood MWAS. Significant overlap was observed between the B-cell MWAS and genetic studies of education attainment and intelligence. Furthermore, the results from both T cells and monocytes overlapped with findings from an MWAS of psychosis conducted in brain tissue. CONCLUSION: In one of the first methylome-wide association studies of adolescent cannabis use, several methylation sites located in genes of importance for potentially relevant brain functions were identified. These findings resulted in several testable hypotheses by which cannabis-associated methylation can impact neurological development and inflammation response as well as potential mechanisms linking cannabis-associated methylation to potential downstream mental health effects.

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Published In

J Am Acad Child Adolesc Psychiatry

DOI

EISSN

1527-5418

Publication Date

December 2021

Volume

60

Issue

12

Start / End Page

1524 / 1532

Location

United States

Related Subject Headings

  • Psychotic Disorders
  • Mental Health
  • Humans
  • Genome-Wide Association Study
  • Developmental & Child Psychology
  • DNA Methylation
  • Cannabis
  • Adolescent
  • 5201 Applied and developmental psychology
  • 3213 Paediatrics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Clark, S. L., Chan, R., Zhao, M., Xie, L. Y., Copeland, W. E., Aberg, K. A., & van den Oord, E. J. C. G. (2021). Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences. J Am Acad Child Adolesc Psychiatry, 60(12), 1524–1532. https://doi.org/10.1016/j.jaac.2021.02.008
Clark, Shaunna L., Robin Chan, Min Zhao, Lin Y. Xie, William E. Copeland, Karolina A. Aberg, and Edwin J. C. G. van den Oord. “Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences.J Am Acad Child Adolesc Psychiatry 60, no. 12 (December 2021): 1524–32. https://doi.org/10.1016/j.jaac.2021.02.008.
Clark SL, Chan R, Zhao M, Xie LY, Copeland WE, Aberg KA, et al. Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences. J Am Acad Child Adolesc Psychiatry. 2021 Dec;60(12):1524–32.
Clark, Shaunna L., et al. “Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences.J Am Acad Child Adolesc Psychiatry, vol. 60, no. 12, Dec. 2021, pp. 1524–32. Pubmed, doi:10.1016/j.jaac.2021.02.008.
Clark SL, Chan R, Zhao M, Xie LY, Copeland WE, Aberg KA, van den Oord EJCG. Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences. J Am Acad Child Adolesc Psychiatry. 2021 Dec;60(12):1524–1532.
Journal cover image

Published In

J Am Acad Child Adolesc Psychiatry

DOI

EISSN

1527-5418

Publication Date

December 2021

Volume

60

Issue

12

Start / End Page

1524 / 1532

Location

United States

Related Subject Headings

  • Psychotic Disorders
  • Mental Health
  • Humans
  • Genome-Wide Association Study
  • Developmental & Child Psychology
  • DNA Methylation
  • Cannabis
  • Adolescent
  • 5201 Applied and developmental psychology
  • 3213 Paediatrics