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mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report.

Publication ,  Journal Article
Venugopal, A; Gillick-Walker, J; Michalczyk, A; Khasraw, M; Ackland, ML
Published in: BMC Gastroenterol
April 27, 2021

BACKGROUND: Neuroendocrine neoplasms (NENs) are a complex group of tumours that occur in many organs. Routinely used IHC markers for NEN diagnosis include CgA, synaptophysin, Ki67 and CD56. These have limitations including lack of correlation to clinical outcomes and their presence in non-tumour tissue. Identification of additional markers and more quantitative analyses of tumour tissue has the potential to contribute to improved clinical outcomes. We used qRT-PCR to profile the expression levels of a panel of markers in tumour and matched non-tumour tissue from a patient with a G1 pancreatic neuroendocrine tumour. Differences in mRNA levels between tumour and non-tumour tissue were compared with IHC analyses of the same sample. CASE PRESENTATION: An elderly man presented with lower abdominal pain for 6 months. Histological analysis identified a low grade, well differentiated pancreatic endocrine neoplasm. Twenty-seven tumour markers for neuroendocrine status, proliferation, stem cell phenotype, angiogenesis, epithelial to mesenchymal transition, cell adhesion, differentiation and tumour suppression were selected from previous studies and mRNA levels of these markers were measured in tumour and adjacent non-tumour tissue sample using qRT-PCR. IHC was carried out on the same tissue to detect the corresponding marker proteins. Of the markers analysed, seven showed higher mRNA levels in tumour relative to non-tumour tissue while thirteen had lower expression in tumour relative to non-tumour tissue. Substantial differences in mRNA levels were a gain of CgA, CD56, β-catenin, CK20, PDX1 and p53 and loss of Ki67, PCAD, CK7, CD31, MENA, ECAD, EPCAM, CDX2 and CK6. Comparison of qRT-PCR data with IHC showed correlation between fifteen markers. CONCLUSION: Our study is unique as it included matched controls that provided a comparative assessment for tumour tissue analysis, whereas many previous studies report tumour data only. Additionally, we utilised qRT-PCR, a relatively quantitative diagnostic tool for differential marker profiling, having the advantage of being reproducible, fast, cheap and accurate. qRT-PCR has the potential to improve the defining of tumour phenotypes and, in combination with IHC may have clinical utility towards improving tumour stratification or distinguishing tumour grades. The results need to be validated with different grades of NENs and related to clinical outcomes.

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Published In

BMC Gastroenterol

DOI

EISSN

1471-230X

Publication Date

April 27, 2021

Volume

21

Issue

1

Start / End Page

194

Location

England

Related Subject Headings

  • RNA, Messenger
  • Neuroendocrine Tumors
  • Male
  • Immunohistochemistry
  • Humans
  • Gastroenterology & Hepatology
  • Epithelial-Mesenchymal Transition
  • Biomarkers, Tumor
  • Aged
  • 3202 Clinical sciences
 

Citation

APA
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MLA
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Venugopal, A., Gillick-Walker, J., Michalczyk, A., Khasraw, M., & Ackland, M. L. (2021). mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report. BMC Gastroenterol, 21(1), 194. https://doi.org/10.1186/s12876-021-01705-9
Venugopal, Abhirami, Jessie Gillick-Walker, Agnes Michalczyk, Mustafa Khasraw, and M Leigh Ackland. “mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report.BMC Gastroenterol 21, no. 1 (April 27, 2021): 194. https://doi.org/10.1186/s12876-021-01705-9.
Venugopal A, Gillick-Walker J, Michalczyk A, Khasraw M, Ackland ML. mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report. BMC Gastroenterol. 2021 Apr 27;21(1):194.
Venugopal, Abhirami, et al. “mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report.BMC Gastroenterol, vol. 21, no. 1, Apr. 2021, p. 194. Pubmed, doi:10.1186/s12876-021-01705-9.
Venugopal A, Gillick-Walker J, Michalczyk A, Khasraw M, Ackland ML. mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report. BMC Gastroenterol. 2021 Apr 27;21(1):194.
Journal cover image

Published In

BMC Gastroenterol

DOI

EISSN

1471-230X

Publication Date

April 27, 2021

Volume

21

Issue

1

Start / End Page

194

Location

England

Related Subject Headings

  • RNA, Messenger
  • Neuroendocrine Tumors
  • Male
  • Immunohistochemistry
  • Humans
  • Gastroenterology & Hepatology
  • Epithelial-Mesenchymal Transition
  • Biomarkers, Tumor
  • Aged
  • 3202 Clinical sciences