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PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.

Publication ,  Journal Article
Leuthner, TC; Hartman, JH; Ryde, IT; Meyer, JN
Published in: Methods in molecular biology (Clifton, N.J.)
January 2021

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for simultaneous isolation of mtDNA and nuclear DNA (nucDNA), and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material, and availability of specific PCR reagents. We also briefly describe factors that affect mtDNA copy number and discuss caveats to its use as a biomarker.

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Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2021

Volume

2310

Start / End Page

91 / 111

Related Subject Headings

  • Zebrafish
  • Saccharomyces cerevisiae
  • Real-Time Polymerase Chain Reaction
  • Rats
  • Oryzias
  • Mitochondria
  • Mice
  • Humans
  • Gene Dosage
  • Fundulidae
 

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Leuthner, T. C., Hartman, J. H., Ryde, I. T., & Meyer, J. N. (2021). PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species. Methods in Molecular Biology (Clifton, N.J.), 2310, 91–111. https://doi.org/10.1007/978-1-0716-1433-4_8
Leuthner, Tess C., Jessica H. Hartman, Ian T. Ryde, and Joel N. Meyer. “PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.Methods in Molecular Biology (Clifton, N.J.) 2310 (January 2021): 91–111. https://doi.org/10.1007/978-1-0716-1433-4_8.
Leuthner TC, Hartman JH, Ryde IT, Meyer JN. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species. Methods in molecular biology (Clifton, NJ). 2021 Jan;2310:91–111.
Leuthner, Tess C., et al. “PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.Methods in Molecular Biology (Clifton, N.J.), vol. 2310, Jan. 2021, pp. 91–111. Epmc, doi:10.1007/978-1-0716-1433-4_8.
Leuthner TC, Hartman JH, Ryde IT, Meyer JN. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species. Methods in molecular biology (Clifton, NJ). 2021 Jan;2310:91–111.

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2021

Volume

2310

Start / End Page

91 / 111

Related Subject Headings

  • Zebrafish
  • Saccharomyces cerevisiae
  • Real-Time Polymerase Chain Reaction
  • Rats
  • Oryzias
  • Mitochondria
  • Mice
  • Humans
  • Gene Dosage
  • Fundulidae