PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.
Publication
, Journal Article
Leuthner, TC; Hartman, JH; Ryde, IT; Meyer, JN
Published in: Methods in molecular biology (Clifton, N.J.)
January 2021
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for simultaneous isolation of mtDNA and nuclear DNA (nucDNA), and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material, and availability of specific PCR reagents. We also briefly describe factors that affect mtDNA copy number and discuss caveats to its use as a biomarker.
Duke Scholars
Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2021
Volume
2310
Start / End Page
91 / 111
Related Subject Headings
- Zebrafish
- Saccharomyces cerevisiae
- Real-Time Polymerase Chain Reaction
- Rats
- Oryzias
- Mitochondria
- Mice
- Humans
- Gene Dosage
- Fundulidae
Citation
APA
Chicago
ICMJE
MLA
NLM
Leuthner, T. C., Hartman, J. H., Ryde, I. T., & Meyer, J. N. (2021). PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species. Methods in Molecular Biology (Clifton, N.J.), 2310, 91–111. https://doi.org/10.1007/978-1-0716-1433-4_8
Leuthner, Tess C., Jessica H. Hartman, Ian T. Ryde, and Joel N. Meyer. “PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.” Methods in Molecular Biology (Clifton, N.J.) 2310 (January 2021): 91–111. https://doi.org/10.1007/978-1-0716-1433-4_8.
Leuthner TC, Hartman JH, Ryde IT, Meyer JN. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species. Methods in molecular biology (Clifton, NJ). 2021 Jan;2310:91–111.
Leuthner, Tess C., et al. “PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.” Methods in Molecular Biology (Clifton, N.J.), vol. 2310, Jan. 2021, pp. 91–111. Epmc, doi:10.1007/978-1-0716-1433-4_8.
Leuthner TC, Hartman JH, Ryde IT, Meyer JN. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species. Methods in molecular biology (Clifton, NJ). 2021 Jan;2310:91–111.
Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2021
Volume
2310
Start / End Page
91 / 111
Related Subject Headings
- Zebrafish
- Saccharomyces cerevisiae
- Real-Time Polymerase Chain Reaction
- Rats
- Oryzias
- Mitochondria
- Mice
- Humans
- Gene Dosage
- Fundulidae