Skip to main content
Journal cover image

Porcine reproductive and respiratory syndrome virus induces pronounced immune modulatory responses at mucosal tissues in the parental vaccine strain VR2332 infected pigs.

Publication ,  Journal Article
Manickam, C; Dwivedi, V; Patterson, R; Papenfuss, T; Renukaradhya, GJ
Published in: Vet Microbiol
February 22, 2013

Porcine reproductive and respiratory syndrome (PRRS) is a chronic viral disease of pigs caused by PRRS virus (PRRSV). The PRRSV VR2332 is the prototype North American parental strain commonly used in the preparation of vaccines. Goal of this study was to understand missing information on VR2332 induced immune modulation at the lungs and lymphoid tissues, the sites of PRRSV replication. Pigs were infected intranasally and samples collected at post-infection day (PID) 15, 30, and 60. Microscopically, lungs had moderate interstitial pneumonia, and the virus was detected in all the tested tissues. Peak antibody response and the cytokine IFN-γ secretion were detected at PID 30, with increased TGF-β until PID 60. Population of CD8(+), CD4(+), and CD4(+)CD8(+)T cells, Natural killer (NK) cells, and γδ T cells in the lungs and lymphoid tissues were significantly modulated favoring PRRSV persistence. The NK cell-mediated cytotoxicity was significantly reduced in infected pigs. In addition, increased population of immunosuppressive T-regulatory cells (Tregs) and associated cytokines were also observed in VR2332 strain infected pigs.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Vet Microbiol

DOI

EISSN

1873-2542

Publication Date

February 22, 2013

Volume

162

Issue

1

Start / End Page

68 / 77

Location

Netherlands

Related Subject Headings

  • Viral Vaccines
  • Viral Load
  • Veterinary Sciences
  • Vaccines, Attenuated
  • Transforming Growth Factor beta
  • Swine
  • Sus scrofa
  • Porcine respiratory and reproductive syndrome virus
  • Porcine Reproductive and Respiratory Syndrome
  • Mucous Membrane
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Manickam, C., Dwivedi, V., Patterson, R., Papenfuss, T., & Renukaradhya, G. J. (2013). Porcine reproductive and respiratory syndrome virus induces pronounced immune modulatory responses at mucosal tissues in the parental vaccine strain VR2332 infected pigs. Vet Microbiol, 162(1), 68–77. https://doi.org/10.1016/j.vetmic.2012.08.021
Manickam, Cordelia, Varun Dwivedi, Ruthi Patterson, Tracey Papenfuss, and Gourapura J. Renukaradhya. “Porcine reproductive and respiratory syndrome virus induces pronounced immune modulatory responses at mucosal tissues in the parental vaccine strain VR2332 infected pigs.Vet Microbiol 162, no. 1 (February 22, 2013): 68–77. https://doi.org/10.1016/j.vetmic.2012.08.021.
Manickam, Cordelia, et al. “Porcine reproductive and respiratory syndrome virus induces pronounced immune modulatory responses at mucosal tissues in the parental vaccine strain VR2332 infected pigs.Vet Microbiol, vol. 162, no. 1, Feb. 2013, pp. 68–77. Pubmed, doi:10.1016/j.vetmic.2012.08.021.
Journal cover image

Published In

Vet Microbiol

DOI

EISSN

1873-2542

Publication Date

February 22, 2013

Volume

162

Issue

1

Start / End Page

68 / 77

Location

Netherlands

Related Subject Headings

  • Viral Vaccines
  • Viral Load
  • Veterinary Sciences
  • Vaccines, Attenuated
  • Transforming Growth Factor beta
  • Swine
  • Sus scrofa
  • Porcine respiratory and reproductive syndrome virus
  • Porcine Reproductive and Respiratory Syndrome
  • Mucous Membrane