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DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.

Publication ,  Journal Article
Fry, AL; Webster, AK; Burnett, J; Chitrakar, R; Baugh, LR; Hubbard, EJA
Published in: PLoS genetics
July 2021

Quiescence, an actively-maintained reversible state of cell cycle arrest, is not well understood. PTEN is one of the most frequently lost tumor suppressors in human cancers and regulates quiescence of stem cells and cancer cells. The sole PTEN ortholog in Caenorhabditis elegans is daf-18. In a C. elegans loss-of-function mutant for daf-18, primordial germ cells (PGCs) divide inappropriately in L1 larvae hatched into starvation conditions, in a TOR-dependent manner. Here, we further investigated the role of daf-18 in maintaining PGC quiescence in L1 starvation. We found that maternal or zygotic daf-18 is sufficient to maintain cell cycle quiescence, that daf-18 acts in the germ line and soma, and that daf-18 affects timing of PGC divisions in fed animals. Importantly, our results also implicate daf-18 in repression of germline zygotic gene activation, though not in germline fate specification. However, TOR is less important to germline zygotic gene expression, suggesting that in the absence of food, daf-18/PTEN prevents inappropriate germline zygotic gene activation and cell division by distinct mechanisms.

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Published In

PLoS genetics

DOI

EISSN

1553-7404

ISSN

1553-7390

Publication Date

July 2021

Volume

17

Issue

7

Start / End Page

e1009650

Related Subject Headings

  • Zygote
  • Transcriptional Activation
  • Signal Transduction
  • PTEN Phosphohydrolase
  • Larva
  • Germ Cells
  • Developmental Biology
  • Cell Proliferation
  • Cell Division
  • Cell Cycle Checkpoints
 

Citation

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ICMJE
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Fry, A. L., Webster, A. K., Burnett, J., Chitrakar, R., Baugh, L. R., & Hubbard, E. J. A. (2021). DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence. PLoS Genetics, 17(7), e1009650. https://doi.org/10.1371/journal.pgen.1009650
Fry, Amanda L., Amy K. Webster, Julia Burnett, Rojin Chitrakar, L Ryan Baugh, and E Jane Albert Hubbard. “DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.PLoS Genetics 17, no. 7 (July 2021): e1009650. https://doi.org/10.1371/journal.pgen.1009650.
Fry AL, Webster AK, Burnett J, Chitrakar R, Baugh LR, Hubbard EJA. DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence. PLoS genetics. 2021 Jul;17(7):e1009650.
Fry, Amanda L., et al. “DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.PLoS Genetics, vol. 17, no. 7, July 2021, p. e1009650. Epmc, doi:10.1371/journal.pgen.1009650.
Fry AL, Webster AK, Burnett J, Chitrakar R, Baugh LR, Hubbard EJA. DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence. PLoS genetics. 2021 Jul;17(7):e1009650.

Published In

PLoS genetics

DOI

EISSN

1553-7404

ISSN

1553-7390

Publication Date

July 2021

Volume

17

Issue

7

Start / End Page

e1009650

Related Subject Headings

  • Zygote
  • Transcriptional Activation
  • Signal Transduction
  • PTEN Phosphohydrolase
  • Larva
  • Germ Cells
  • Developmental Biology
  • Cell Proliferation
  • Cell Division
  • Cell Cycle Checkpoints