Native-type DHP-sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac alpha 1 subunits expressed in Xenopus laevis oocytes and are regulated by alpha 2- and beta-subunits.
Publication
, Journal Article
Itagaki, K; Koch, WJ; Bodi, I; Klöckner, U; Slish, DF; Schwartz, A
Published in: FEBS Lett
February 10, 1992
Native tissue-like L-type voltage-dependent calcium channels (L-VDCC's) were expressed by in vitro transcribed cRNA injection of rat aorta or rabbit cardiac alpha 1 subunit into Xenopus laevis oocytes. Co-injection of VSM-alpha 1 with the cloned skeletal muscle beta-subunit (SK-beta) of the L-type VDCC significantly increased the expressed peak current amplitude without significant changes in kinetics. Similar results were obtained by co-injection of cardiac alpha 1 (DSHT-alpha 1) the cloned skeletal alpha 2-subunit (SK-alpha 2) or with SK-beta. The oocytes co-expressing cRNA's retained L-type VDCC pharmacology.
Duke Scholars
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
February 10, 1992
Volume
297
Issue
3
Start / End Page
221 / 225
Location
England
Related Subject Headings
- Xenopus laevis
- Rats
- Rabbits
- Oocytes
- Myocardium
- Muscle, Smooth, Vascular
- Gene Expression Regulation
- Female
- Electric Conductivity
- Dihydropyridines
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Itagaki, K., Koch, W. J., Bodi, I., Klöckner, U., Slish, D. F., & Schwartz, A. (1992). Native-type DHP-sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac alpha 1 subunits expressed in Xenopus laevis oocytes and are regulated by alpha 2- and beta-subunits. FEBS Lett, 297(3), 221–225. https://doi.org/10.1016/0014-5793(92)80542-o
Itagaki, K., W. J. Koch, I. Bodi, U. Klöckner, D. F. Slish, and A. Schwartz. “Native-type DHP-sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac alpha 1 subunits expressed in Xenopus laevis oocytes and are regulated by alpha 2- and beta-subunits.” FEBS Lett 297, no. 3 (February 10, 1992): 221–25. https://doi.org/10.1016/0014-5793(92)80542-o.
Itagaki K, Koch WJ, Bodi I, Klöckner U, Slish DF, Schwartz A. Native-type DHP-sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac alpha 1 subunits expressed in Xenopus laevis oocytes and are regulated by alpha 2- and beta-subunits. FEBS Lett. 1992 Feb 10;297(3):221–5.
Itagaki, K., et al. “Native-type DHP-sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac alpha 1 subunits expressed in Xenopus laevis oocytes and are regulated by alpha 2- and beta-subunits.” FEBS Lett, vol. 297, no. 3, Feb. 1992, pp. 221–25. Pubmed, doi:10.1016/0014-5793(92)80542-o.
Itagaki K, Koch WJ, Bodi I, Klöckner U, Slish DF, Schwartz A. Native-type DHP-sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac alpha 1 subunits expressed in Xenopus laevis oocytes and are regulated by alpha 2- and beta-subunits. FEBS Lett. 1992 Feb 10;297(3):221–225.
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
February 10, 1992
Volume
297
Issue
3
Start / End Page
221 / 225
Location
England
Related Subject Headings
- Xenopus laevis
- Rats
- Rabbits
- Oocytes
- Myocardium
- Muscle, Smooth, Vascular
- Gene Expression Regulation
- Female
- Electric Conductivity
- Dihydropyridines