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The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.

Publication ,  Journal Article
Spencer, DM; Reyna, AG; Pisetsky, DS
Published in: International journal of molecular sciences
August 2021

DNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is considered to be transient. To determine whether the presence of Z-DNA can be detected immunochemically, the binding of monoclonal and polyclonal anti-Z-DNA antibodies to a panel of natural DNA antigens was assessed by an ELISA using brominated poly(dG-dC) as a control for Z-DNA. As these studies showed, among natural DNA tested (Micrococcus luteus, calf thymus, Escherichiacoli, salmon sperm, lambda phage), micrococcal (MC) DNA showed the highest binding with both anti-Z-DNA preparations, and E. coli DNA showed binding with the monoclonal anti-DNA preparation. The specificity for Z-DNA conformation in MC DNA was demonstrated by an inhibition binding assay. An algorithm to identify propensity to form Z-DNA indicated that DNA from Mycobacterium tuberculosis could form Z-DNA, a prediction confirmed by immunoassay. Together, these findings indicate that anti-Z-DNA antibodies can serve as probes for the presence of Z-DNA in DNA of various species origin and that the content of Z-DNA varies significantly among DNA sources.

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Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

August 2021

Volume

22

Issue

16

Start / End Page

8931

Related Subject Headings

  • Spermatozoa
  • Species Specificity
  • Sheep
  • Salmon
  • Pregnancy
  • Placenta
  • Nucleic Acid Conformation
  • Micrococcus luteus
  • Male
  • Humans
 

Citation

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ICMJE
MLA
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Spencer, D. M., Reyna, A. G., & Pisetsky, D. S. (2021). The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin. International Journal of Molecular Sciences, 22(16), 8931. https://doi.org/10.3390/ijms22168931
Spencer, Diane M., Angel Garza Reyna, and David S. Pisetsky. “The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.International Journal of Molecular Sciences 22, no. 16 (August 2021): 8931. https://doi.org/10.3390/ijms22168931.
Spencer DM, Reyna AG, Pisetsky DS. The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin. International journal of molecular sciences. 2021 Aug;22(16):8931.
Spencer, Diane M., et al. “The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.International Journal of Molecular Sciences, vol. 22, no. 16, Aug. 2021, p. 8931. Epmc, doi:10.3390/ijms22168931.
Spencer DM, Reyna AG, Pisetsky DS. The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin. International journal of molecular sciences. 2021 Aug;22(16):8931.

Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

August 2021

Volume

22

Issue

16

Start / End Page

8931

Related Subject Headings

  • Spermatozoa
  • Species Specificity
  • Sheep
  • Salmon
  • Pregnancy
  • Placenta
  • Nucleic Acid Conformation
  • Micrococcus luteus
  • Male
  • Humans