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The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding.

Publication ,  Journal Article
Pisetsky, DS; Shaffer, R; Armstrong, DD; Spencer, DM
Published in: Clin Immunol
October 2021

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus. Previous studies have indicated that the phosphodiester backbone is the main antigenic target, with electrostatic interactions important for high avidity. To define further these interactions, the effects of ionic strength on anti-DNA binding of SLE plasmas were assessed in association and dissociation assays by ELISA. As these studies demonstrated, increasing ionic strength to a concentration of 1000 mM NaCl reduced antibody binding although the extent of the reduction varied among samples. In dissociation assays, differences among plasmas were also observed. For one of the plasmas, binding to DNA displayed resistance to dissociation by increasing ionic strength even though these concentrations limited binding in association assays. Time course studies showed a gradual change in binding interactions. These studies indicate that anti-DNA binding can involve both electrostatic and non-electrostatic interactions, with binding in some plasmas showing evidence of hysteresis.

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Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

October 2021

Volume

231

Start / End Page

108848

Location

United States

Related Subject Headings

  • Osmolar Concentration
  • Lupus Erythematosus, Systemic
  • Immunology
  • Humans
  • DNA
  • Autoantigens
  • Antigen-Antibody Complex
  • Antibody Affinity
  • Antibodies, Antinuclear
  • 3204 Immunology
 

Citation

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ICMJE
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Pisetsky, D. S., Shaffer, R., Armstrong, D. D., & Spencer, D. M. (2021). The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding. Clin Immunol, 231, 108848. https://doi.org/10.1016/j.clim.2021.108848
Pisetsky, David S., Robert Shaffer, Dustin D. Armstrong, and Diane M. Spencer. “The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding.Clin Immunol 231 (October 2021): 108848. https://doi.org/10.1016/j.clim.2021.108848.
Pisetsky DS, Shaffer R, Armstrong DD, Spencer DM. The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding. Clin Immunol. 2021 Oct;231:108848.
Pisetsky, David S., et al. “The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding.Clin Immunol, vol. 231, Oct. 2021, p. 108848. Pubmed, doi:10.1016/j.clim.2021.108848.
Pisetsky DS, Shaffer R, Armstrong DD, Spencer DM. The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding. Clin Immunol. 2021 Oct;231:108848.
Journal cover image

Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

October 2021

Volume

231

Start / End Page

108848

Location

United States

Related Subject Headings

  • Osmolar Concentration
  • Lupus Erythematosus, Systemic
  • Immunology
  • Humans
  • DNA
  • Autoantigens
  • Antigen-Antibody Complex
  • Antibody Affinity
  • Antibodies, Antinuclear
  • 3204 Immunology