Skip to main content

Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment.

Publication ,  Journal Article
Liu, H; Bai, H; Hui, E; Yang, L; Evans, CS; Wang, Z; Kwon, SE; Chapman, ER
Published in: Elife
February 25, 2014

Synaptotagmin (syt) 7 is one of three syt isoforms found in all metazoans; it is ubiquitously expressed, yet its function in neurons remains obscure. Here, we resolved Ca(2+)-dependent and Ca(2+)-independent synaptic vesicle (SV) replenishment pathways, and found that syt 7 plays a selective and critical role in the Ca(2+)-dependent pathway. Mutations that disrupt Ca(2+)-binding to syt 7 abolish this function, suggesting that syt 7 functions as a Ca(2+)-sensor for replenishment. The Ca(2+)-binding protein calmodulin (CaM) has also been implicated in SV replenishment, and we found that loss of syt 7 was phenocopied by a CaM antagonist. Moreover, we discovered that syt 7 binds to CaM in a highly specific and Ca(2+)-dependent manner; this interaction requires intact Ca(2+)-binding sites within syt 7. Together, these data indicate that a complex of two conserved Ca(2+)-binding proteins, syt 7 and CaM, serve as a key regulator of SV replenishment in presynaptic nerve terminals. DOI: http://dx.doi.org/10.7554/eLife.01524.001.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

February 25, 2014

Volume

3

Start / End Page

e01524

Location

England

Related Subject Headings

  • Time Factors
  • Synaptotagmins
  • Synaptic Vesicles
  • Synaptic Transmission
  • Protein Interaction Domains and Motifs
  • Protein Binding
  • Presynaptic Terminals
  • Neuronal Plasticity
  • Mice, Knockout
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Liu, H., Bai, H., Hui, E., Yang, L., Evans, C. S., Wang, Z., … Chapman, E. R. (2014). Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment. Elife, 3, e01524. https://doi.org/10.7554/eLife.01524
Liu, Huisheng, Hua Bai, Enfu Hui, Lu Yang, Chantell S. Evans, Zhao Wang, Sung E. Kwon, and Edwin R. Chapman. “Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment.Elife 3 (February 25, 2014): e01524. https://doi.org/10.7554/eLife.01524.
Liu H, Bai H, Hui E, Yang L, Evans CS, Wang Z, et al. Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment. Elife. 2014 Feb 25;3:e01524.
Liu, Huisheng, et al. “Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment.Elife, vol. 3, Feb. 2014, p. e01524. Pubmed, doi:10.7554/eLife.01524.
Liu H, Bai H, Hui E, Yang L, Evans CS, Wang Z, Kwon SE, Chapman ER. Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment. Elife. 2014 Feb 25;3:e01524.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

February 25, 2014

Volume

3

Start / End Page

e01524

Location

England

Related Subject Headings

  • Time Factors
  • Synaptotagmins
  • Synaptic Vesicles
  • Synaptic Transmission
  • Protein Interaction Domains and Motifs
  • Protein Binding
  • Presynaptic Terminals
  • Neuronal Plasticity
  • Mice, Knockout
  • Mice