Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer.
Publication
, Journal Article
Chan, FK-M
Published in: Methods Mol Biol
2004
Variants of the green fluorescence protein (GFP) are useful tools in many biological research applications. Two of the GFP spectral variants, the cyan and yellow fluorescence proteins (CFP and YFP), have compatible excitation and emission properties as fluorescence donor and acceptor in fluorescence resonance energy transfer (FRET). A protocol is described here to study the molecular association between p80 TNFR-2 and TRAF2 fusion proteins containing CFP and YFP using flow cytometric FRET measurement. The utility of this application in the study of protein-protein interaction is discussed.
Duke Scholars
Published In
Methods Mol Biol
DOI
ISSN
1064-3745
Publication Date
2004
Volume
261
Start / End Page
371 / 382
Location
United States
Related Subject Headings
- TNF Receptor-Associated Factor 2
- Receptors, Tumor Necrosis Factor, Type II
- Receptors, Tumor Necrosis Factor
- Proteins
- Protein Interaction Mapping
- Protein Binding
- Luminescent Proteins
- Humans
- Green Fluorescent Proteins
- Fluorescence Resonance Energy Transfer
Citation
APA
Chicago
ICMJE
MLA
NLM
Chan, F.-M. (2004). Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer. Methods Mol Biol, 261, 371–382. https://doi.org/10.1385/1-59259-762-9:371
Chan, Francis Ka-Ming. “Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer.” Methods Mol Biol 261 (2004): 371–82. https://doi.org/10.1385/1-59259-762-9:371.
Chan FK-M. Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer. Methods Mol Biol. 2004;261:371–82.
Chan, Francis Ka-Ming. “Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer.” Methods Mol Biol, vol. 261, 2004, pp. 371–82. Pubmed, doi:10.1385/1-59259-762-9:371.
Chan FK-M. Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer. Methods Mol Biol. 2004;261:371–382.
Published In
Methods Mol Biol
DOI
ISSN
1064-3745
Publication Date
2004
Volume
261
Start / End Page
371 / 382
Location
United States
Related Subject Headings
- TNF Receptor-Associated Factor 2
- Receptors, Tumor Necrosis Factor, Type II
- Receptors, Tumor Necrosis Factor
- Proteins
- Protein Interaction Mapping
- Protein Binding
- Luminescent Proteins
- Humans
- Green Fluorescent Proteins
- Fluorescence Resonance Energy Transfer