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SEMA3A signaling controls layer-specific interneuron branching in the cerebellum.

Publication ,  Journal Article
Cioni, J-M; Telley, L; Saywell, V; Cadilhac, C; Jourdan, C; Huber, AB; Huang, JZ; Jahannault-Talignani, C; Ango, F
Published in: Curr Biol
May 20, 2013

BACKGROUND: GABAergic interneurons regulate the balance and dynamics of neural circuits, in part, by elaborating their strategically placed axon branches that innervate specific cellular and subcellular targets. However, the molecular mechanisms that regulate target-directed GABAergic axon branching are not well understood. RESULTS: Here we show that the secreted axon guidance molecule, SEMA3A, expressed locally by Purkinje cells, regulates cerebellar basket cell axon branching through its cognate receptor Neuropilin-1 (NRP1). SEMA3A was specifically localized and enriched in the Purkinje cell layer (PCL). In sema3A(-/-) and nrp1(sema-/sema-) mice lacking SEMA3A-binding domains, basket axon branching in PCL was reduced. We demonstrate that SEMA3A-induced axon branching was dependent on local recruitment of soluble guanylyl cyclase (sGC) to the plasma membrane of basket cells, and sGC subcellular trafficking was regulated by the Src kinase FYN. In fyn-deficient mice, basket axon terminal branching was reduced in PCL, but not in the molecular layer. CONCLUSIONS: These results demonstrate a critical role of local SEMA3A signaling in layer-specific axonal branching, which contributes to target innervation.

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Published In

Curr Biol

DOI

EISSN

1879-0445

Publication Date

May 20, 2013

Volume

23

Issue

10

Start / End Page

850 / 861

Location

England

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Signal Transduction
  • Semaphorin-3A
  • Protein Transport
  • Mice, Knockout
  • Mice
  • Interneurons
  • Guanylate Cyclase
  • Developmental Biology
  • Cyclic GMP
 

Citation

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Chicago
ICMJE
MLA
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Cioni, J.-M., Telley, L., Saywell, V., Cadilhac, C., Jourdan, C., Huber, A. B., … Ango, F. (2013). SEMA3A signaling controls layer-specific interneuron branching in the cerebellum. Curr Biol, 23(10), 850–861. https://doi.org/10.1016/j.cub.2013.04.007
Cioni, Jean-Michel, Ludovic Telley, Véronique Saywell, Christelle Cadilhac, Carole Jourdan, Andrea B. Huber, Josh Z. Huang, Céline Jahannault-Talignani, and Fabrice Ango. “SEMA3A signaling controls layer-specific interneuron branching in the cerebellum.Curr Biol 23, no. 10 (May 20, 2013): 850–61. https://doi.org/10.1016/j.cub.2013.04.007.
Cioni J-M, Telley L, Saywell V, Cadilhac C, Jourdan C, Huber AB, et al. SEMA3A signaling controls layer-specific interneuron branching in the cerebellum. Curr Biol. 2013 May 20;23(10):850–61.
Cioni, Jean-Michel, et al. “SEMA3A signaling controls layer-specific interneuron branching in the cerebellum.Curr Biol, vol. 23, no. 10, May 2013, pp. 850–61. Pubmed, doi:10.1016/j.cub.2013.04.007.
Cioni J-M, Telley L, Saywell V, Cadilhac C, Jourdan C, Huber AB, Huang JZ, Jahannault-Talignani C, Ango F. SEMA3A signaling controls layer-specific interneuron branching in the cerebellum. Curr Biol. 2013 May 20;23(10):850–861.
Journal cover image

Published In

Curr Biol

DOI

EISSN

1879-0445

Publication Date

May 20, 2013

Volume

23

Issue

10

Start / End Page

850 / 861

Location

England

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Signal Transduction
  • Semaphorin-3A
  • Protein Transport
  • Mice, Knockout
  • Mice
  • Interneurons
  • Guanylate Cyclase
  • Developmental Biology
  • Cyclic GMP