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Targeting cells with single vectors using multiple-feature Boolean logic.

Publication ,  Journal Article
Fenno, LE; Mattis, J; Ramakrishnan, C; Hyun, M; Lee, SY; He, M; Tucciarone, J; Selimbeyoglu, A; Berndt, A; Grosenick, L; Zalocusky, KA ...
Published in: Nat Methods
July 2014

Precisely defining the roles of specific cell types is an intriguing frontier in the study of intact biological systems and has stimulated the rapid development of genetically encoded tools for observation and control. However, targeting these tools with adequate specificity remains challenging: most cell types are best defined by the intersection of two or more features such as active promoter elements, location and connectivity. Here we have combined engineered introns with specific recombinases to achieve expression of genetically encoded tools that is conditional upon multiple cell-type features, using Boolean logical operations all governed by a single versatile vector. We used this approach to target intersectionally specified populations of inhibitory interneurons in mammalian hippocampus and neurons of the ventral tegmental area defined by both genetic and wiring properties. This flexible and modular approach may expand the application of genetically encoded interventional and observational tools for intact-systems biology.

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Published In

Nat Methods

DOI

EISSN

1548-7105

Publication Date

July 2014

Volume

11

Issue

7

Start / End Page

763 / 772

Location

United States

Related Subject Headings

  • Transgenes
  • Promoter Regions, Genetic
  • Mice, Transgenic
  • Mice
  • Male
  • Luminescent Proteins
  • Logic
  • Introns
  • Interneurons
  • Integrases
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fenno, L. E., Mattis, J., Ramakrishnan, C., Hyun, M., Lee, S. Y., He, M., … Deisseroth, K. (2014). Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods, 11(7), 763–772. https://doi.org/10.1038/nmeth.2996
Fenno, Lief E., Joanna Mattis, Charu Ramakrishnan, Minsuk Hyun, Soo Yeun Lee, Miao He, Jason Tucciarone, et al. “Targeting cells with single vectors using multiple-feature Boolean logic.Nat Methods 11, no. 7 (July 2014): 763–72. https://doi.org/10.1038/nmeth.2996.
Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, et al. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods. 2014 Jul;11(7):763–72.
Fenno, Lief E., et al. “Targeting cells with single vectors using multiple-feature Boolean logic.Nat Methods, vol. 11, no. 7, July 2014, pp. 763–72. Pubmed, doi:10.1038/nmeth.2996.
Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, Deisseroth K. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods. 2014 Jul;11(7):763–772.

Published In

Nat Methods

DOI

EISSN

1548-7105

Publication Date

July 2014

Volume

11

Issue

7

Start / End Page

763 / 772

Location

United States

Related Subject Headings

  • Transgenes
  • Promoter Regions, Genetic
  • Mice, Transgenic
  • Mice
  • Male
  • Luminescent Proteins
  • Logic
  • Introns
  • Interneurons
  • Integrases