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Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling.

Publication ,  Journal Article
Chen, Z; Hankey, W; Zhao, Y; Groth, J; Huang, F; Wang, H; Campos, AR; Huang, J; Roeder, RG; Wang, Q
Published in: Nat Commun
November 3, 2021

RNA Polymerase II (Pol II) transcriptional recycling is a mechanism for which the required factors and contributions to overall gene expression levels are poorly understood. We describe an in vitro methodology facilitating unbiased identification of putative RNA Pol II transcriptional recycling factors and quantitative measurement of transcriptional output from recycled transcriptional components. Proof-of-principle experiments identified PAF1 complex components among recycling factors and detected defective transcriptional output from Pol II recycling following PAF1 depletion. Dynamic ChIP-seq confirmed PAF1 silencing triggered defective Pol II recycling in human cells. Prostate tumors exhibited enhanced transcriptional recycling, which was attenuated by antibody-based PAF1 depletion. These findings identify Pol II recycling as a potential target in cancer and demonstrate the applicability of in vitro and cellular transcription assays to characterize Pol II recycling in other disease states.

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

November 3, 2021

Volume

12

Issue

1

Start / End Page

6318

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • RNA Polymerase II
  • Nuclear Proteins
  • Neoplasms
  • Male
  • Humans
  • Hela Cells
  • HeLa Cells
  • Cell Line, Tumor
 

Citation

APA
Chicago
ICMJE
MLA
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Chen, Z., Hankey, W., Zhao, Y., Groth, J., Huang, F., Wang, H., … Wang, Q. (2021). Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling. Nat Commun, 12(1), 6318. https://doi.org/10.1038/s41467-021-26604-1
Chen, Zhong, William Hankey, Yue Zhao, Jeff Groth, Furong Huang, Hongyan Wang, Alexandre Rosa Campos, Jiaoti Huang, Robert G. Roeder, and Qianben Wang. “Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling.Nat Commun 12, no. 1 (November 3, 2021): 6318. https://doi.org/10.1038/s41467-021-26604-1.
Chen Z, Hankey W, Zhao Y, Groth J, Huang F, Wang H, et al. Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling. Nat Commun. 2021 Nov 3;12(1):6318.
Chen, Zhong, et al. “Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling.Nat Commun, vol. 12, no. 1, Nov. 2021, p. 6318. Pubmed, doi:10.1038/s41467-021-26604-1.
Chen Z, Hankey W, Zhao Y, Groth J, Huang F, Wang H, Campos AR, Huang J, Roeder RG, Wang Q. Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling. Nat Commun. 2021 Nov 3;12(1):6318.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

November 3, 2021

Volume

12

Issue

1

Start / End Page

6318

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • RNA Polymerase II
  • Nuclear Proteins
  • Neoplasms
  • Male
  • Humans
  • Hela Cells
  • HeLa Cells
  • Cell Line, Tumor