Peptide binding proclivities of calcium loaded calbindin-D28k.
Publication
, Journal Article
Kordys, DR; Bobay, BG; Thompson, RJ; Venters, RA; Cavanagh, J
Published in: FEBS Lett
October 2, 2007
Calbindin-D28k is known to function as a calcium-buffering protein in the cell. Moreover, recent evidence shows that it also plays a role as a sensor. Using circular dichroism and NMR, we show that calbindin-D28k undergoes significant conformational changes upon binding calcium, whereas only minor changes occur when binding target peptides in its Ca(2+)-loaded state. NMR experiments also identify residues that undergo chemical shift changes as a result of peptide binding. The subsequent use of computational protein-protein docking protocols produce a model describing the interaction interface between calbindin-D28k and its target peptides.
Duke Scholars
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
October 2, 2007
Volume
581
Issue
24
Start / End Page
4778 / 4782
Location
England
Related Subject Headings
- Titrimetry
- S100 Calcium Binding Protein G
- Rats
- Protein Structure, Tertiary
- Protein Binding
- Peptides
- Nuclear Magnetic Resonance, Biomolecular
- Models, Molecular
- Circular Dichroism
- Calcium
Citation
APA
Chicago
ICMJE
MLA
NLM
Kordys, D. R., Bobay, B. G., Thompson, R. J., Venters, R. A., & Cavanagh, J. (2007). Peptide binding proclivities of calcium loaded calbindin-D28k. FEBS Lett, 581(24), 4778–4782. https://doi.org/10.1016/j.febslet.2007.09.004
Kordys, David R., Benjamin G. Bobay, Richele J. Thompson, Ronald A. Venters, and John Cavanagh. “Peptide binding proclivities of calcium loaded calbindin-D28k.” FEBS Lett 581, no. 24 (October 2, 2007): 4778–82. https://doi.org/10.1016/j.febslet.2007.09.004.
Kordys DR, Bobay BG, Thompson RJ, Venters RA, Cavanagh J. Peptide binding proclivities of calcium loaded calbindin-D28k. FEBS Lett. 2007 Oct 2;581(24):4778–82.
Kordys, David R., et al. “Peptide binding proclivities of calcium loaded calbindin-D28k.” FEBS Lett, vol. 581, no. 24, Oct. 2007, pp. 4778–82. Pubmed, doi:10.1016/j.febslet.2007.09.004.
Kordys DR, Bobay BG, Thompson RJ, Venters RA, Cavanagh J. Peptide binding proclivities of calcium loaded calbindin-D28k. FEBS Lett. 2007 Oct 2;581(24):4778–4782.
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
October 2, 2007
Volume
581
Issue
24
Start / End Page
4778 / 4782
Location
England
Related Subject Headings
- Titrimetry
- S100 Calcium Binding Protein G
- Rats
- Protein Structure, Tertiary
- Protein Binding
- Peptides
- Nuclear Magnetic Resonance, Biomolecular
- Models, Molecular
- Circular Dichroism
- Calcium