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Induction of preneoplastic lung lesions in guinea pigs by cigarette smoke inhalation and their exacerbation by high dietary levels of vitamins C and E.

Publication ,  Journal Article
Fiala, ES; Sohn, OS; Wang, C-X; Seibert, E; Tsurutani, J; Dennis, PA; El-Bayoumy, K; Sodum, RS; Desai, D; Reinhardt, J; Aliaga, C
Published in: Carcinogenesis
March 2005

The development of effective chemopreventive agents against cigarette smoke-induced lung cancer could be greatly facilitated by the availability of suitable laboratory animal models. Here we report that male Hartley guinea pigs treated with cigarette smoke by inhalation twice a day for 28 days developed preneoplastic lung lesions, including bronchial hyperplasia, dysplasia and squamous metaplasia, analogous to those found in human smokers. The lesions were accompanied by increased expression of proliferating cell nuclear antigen and activation of the serine/threonine kinase Akt in the bronchial epithelium. In contrast, no lung lesions were found in guinea pigs ('sham smoked') that were submitted to identical procedures but without cigarettes. Compared with a diet low in vitamin C (50 p.p.m.) and vitamin E (15 p.p.m.), a diet high in vitamin C (4000 p.p.m.) and vitamin E (40 p.p.m.) significantly increased the incidence of these lesions. The inclusion of 1,4-phenylenebis(methylene)selenocyanate (p-XSC), a synthetic chemopreventive organoselenium compound, in the high vitamin C-high vitamin E diet at a level of 15 p.p.m. as selenium appeared to decrease the lesion incidence. Administration of (-)-epigallocatechin gallate, a powerful green tea polyphenolic antioxidant, at 560 p.p.m. in the drinking water had no effect. As in human smokers, levels of ascorbate in blood plasma, lung, liver and the adrenal glands were significantly decreased by cigarette smoke inhalation. These results identify a relevant in vivo laboratory model of cigarette smoke-induced lung cancer, suggest that p-XSC may have activity as a chemopreventive agent against cigarette smoke-induced lung lesions and provide additional evidence that very high dietary levels of certain antioxidants can have co-carcinogenic activity in cigarette smoke-induced lung cancer.

Duke Scholars

Published In

Carcinogenesis

DOI

ISSN

0143-3334

Publication Date

March 2005

Volume

26

Issue

3

Start / End Page

605 / 612

Location

England

Related Subject Headings

  • Vitamin E
  • Smoke
  • Proto-Oncogene Proteins c-akt
  • Proto-Oncogene Proteins
  • Protein Serine-Threonine Kinases
  • Proliferating Cell Nuclear Antigen
  • Precancerous Conditions
  • Oncology & Carcinogenesis
  • Nicotiana
  • Lung Neoplasms
 

Citation

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Fiala, E. S., Sohn, O. S., Wang, C.-X., Seibert, E., Tsurutani, J., Dennis, P. A., … Aliaga, C. (2005). Induction of preneoplastic lung lesions in guinea pigs by cigarette smoke inhalation and their exacerbation by high dietary levels of vitamins C and E. Carcinogenesis, 26(3), 605–612. https://doi.org/10.1093/carcin/bgh341
Fiala, Emerich S., Ock Soon Sohn, Chung-Xiou Wang, Eleanore Seibert, Junji Tsurutani, Phillip A. Dennis, Karam El-Bayoumy, et al. “Induction of preneoplastic lung lesions in guinea pigs by cigarette smoke inhalation and their exacerbation by high dietary levels of vitamins C and E.Carcinogenesis 26, no. 3 (March 2005): 605–12. https://doi.org/10.1093/carcin/bgh341.
Fiala ES, Sohn OS, Wang C-X, Seibert E, Tsurutani J, Dennis PA, et al. Induction of preneoplastic lung lesions in guinea pigs by cigarette smoke inhalation and their exacerbation by high dietary levels of vitamins C and E. Carcinogenesis. 2005 Mar;26(3):605–12.
Fiala, Emerich S., et al. “Induction of preneoplastic lung lesions in guinea pigs by cigarette smoke inhalation and their exacerbation by high dietary levels of vitamins C and E.Carcinogenesis, vol. 26, no. 3, Mar. 2005, pp. 605–12. Pubmed, doi:10.1093/carcin/bgh341.
Fiala ES, Sohn OS, Wang C-X, Seibert E, Tsurutani J, Dennis PA, El-Bayoumy K, Sodum RS, Desai D, Reinhardt J, Aliaga C. Induction of preneoplastic lung lesions in guinea pigs by cigarette smoke inhalation and their exacerbation by high dietary levels of vitamins C and E. Carcinogenesis. 2005 Mar;26(3):605–612.
Journal cover image

Published In

Carcinogenesis

DOI

ISSN

0143-3334

Publication Date

March 2005

Volume

26

Issue

3

Start / End Page

605 / 612

Location

England

Related Subject Headings

  • Vitamin E
  • Smoke
  • Proto-Oncogene Proteins c-akt
  • Proto-Oncogene Proteins
  • Protein Serine-Threonine Kinases
  • Proliferating Cell Nuclear Antigen
  • Precancerous Conditions
  • Oncology & Carcinogenesis
  • Nicotiana
  • Lung Neoplasms