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Studies on the role of basic fibroblast growth factor in vivo: inability of neutralizing antibodies to block tumor growth.

Publication ,  Journal Article
Dennis, PA; Rifkin, DB
Published in: J Cell Physiol
July 1990

Affinity-purified polyclonal rabbit antibodies prepared against recombinant basic fibroblast growth factor (bFGF) neutralized the ability of bFGF to stimulate plasminogen activator (PA) production and endothelial cell migration in vitro. After iodination and intraperitoneal injection of the antibodies in mice, approximately 76% of the maximum circulating level of 125I-anti-bFGF antibodies (AF) was found as intact IgG after 24 hr. Furthermore, the circulating 125I-AF retained the ability to bind bFGF. Studies were performed to determine whether the growth of three different murine tumors (CT26, EHS, or B16/BL6) could be inhibited with affinity-purified neutralizing antibodies against bFGF. Tumors were injected subcutaneously in syngeneic mice, and neutralizing antibodies against bFGF were injected daily into the peritoneum. All studies, which varied in tumor burden, antibody dose, and study length, indicated that neutralizing antibodies against bFGF had no effect on tumor size, tumor growth, or tumor histology.

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Published In

J Cell Physiol

DOI

ISSN

0021-9541

Publication Date

July 1990

Volume

144

Issue

1

Start / End Page

84 / 98

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Neoplasms, Experimental
  • Mice
  • In Vitro Techniques
  • Immunologic Techniques
  • Fibroblast Growth Factors
  • Cell Division
  • Biochemistry & Molecular Biology
  • Animals
  • 3208 Medical physiology
 

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Dennis, P. A., & Rifkin, D. B. (1990). Studies on the role of basic fibroblast growth factor in vivo: inability of neutralizing antibodies to block tumor growth. J Cell Physiol, 144(1), 84–98. https://doi.org/10.1002/jcp.1041440112
Dennis, P. A., and D. B. Rifkin. “Studies on the role of basic fibroblast growth factor in vivo: inability of neutralizing antibodies to block tumor growth.J Cell Physiol 144, no. 1 (July 1990): 84–98. https://doi.org/10.1002/jcp.1041440112.
Dennis, P. A., and D. B. Rifkin. “Studies on the role of basic fibroblast growth factor in vivo: inability of neutralizing antibodies to block tumor growth.J Cell Physiol, vol. 144, no. 1, July 1990, pp. 84–98. Pubmed, doi:10.1002/jcp.1041440112.
Journal cover image

Published In

J Cell Physiol

DOI

ISSN

0021-9541

Publication Date

July 1990

Volume

144

Issue

1

Start / End Page

84 / 98

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Neoplasms, Experimental
  • Mice
  • In Vitro Techniques
  • Immunologic Techniques
  • Fibroblast Growth Factors
  • Cell Division
  • Biochemistry & Molecular Biology
  • Animals
  • 3208 Medical physiology