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Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype.

Publication ,  Journal Article
Swartz, AM; Hotchkiss, KM; Nair, SK; Sampson, JH; Batich, KA
Published in: Methods in molecular biology (Clifton, N.J.)
January 2022

Our group has employed methodologies for effective ex vivo generation of dendritic cell (DC) vaccines for patients with primary malignant brain tumors. In order to reliably produce the most potent, most representational vaccinated DC that will engender an antitumor response requires the ability to orchestrate multiple methodologies that address antigen cross-presentation, T-cell costimulation and polarization, and migratory capacity. In this chapter, we describe a novel method for augmenting the immunogenicity and migratory potential of DCs for their use as vaccines. We have elucidated methodologies to avoid the phenomenon known as immunodominance in generating cancer vaccines. We have found that culturing DC progenitors in serum-free conditions for the duration of the differentiation protocol results in a more homogeneously mature population of DCs that exhibit enhanced immunogenicity compared to DCs generated in serum-containing culture conditions. Furthermore, we demonstrate our method for generating high mobility DCs that readily migrate toward lymphoid organ chemoattractants using CCL3 protein. The combination of these two approaches represents a facile and clinically tractable methodology for generating highly mature DCs with excellent migratory capacity.

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Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2022

Volume

2410

Start / End Page

609 / 626

Related Subject Headings

  • Vaccines
  • Phenotype
  • Neoplasms
  • Humans
  • Developmental Biology
  • Dendritic Cells
  • Cross-Priming
  • Cell Differentiation
  • Cancer Vaccines
  • 3404 Medicinal and biomolecular chemistry
 

Citation

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Swartz, A. M., Hotchkiss, K. M., Nair, S. K., Sampson, J. H., & Batich, K. A. (2022). Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype. Methods in Molecular Biology (Clifton, N.J.), 2410, 609–626. https://doi.org/10.1007/978-1-0716-1884-4_33
Swartz, Adam M., Kelly M. Hotchkiss, Smita K. Nair, John H. Sampson, and Kristen A. Batich. “Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype.Methods in Molecular Biology (Clifton, N.J.) 2410 (January 2022): 609–26. https://doi.org/10.1007/978-1-0716-1884-4_33.
Swartz AM, Hotchkiss KM, Nair SK, Sampson JH, Batich KA. Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype. Methods in molecular biology (Clifton, NJ). 2022 Jan;2410:609–26.
Swartz, Adam M., et al. “Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype.Methods in Molecular Biology (Clifton, N.J.), vol. 2410, Jan. 2022, pp. 609–26. Epmc, doi:10.1007/978-1-0716-1884-4_33.
Swartz AM, Hotchkiss KM, Nair SK, Sampson JH, Batich KA. Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype. Methods in molecular biology (Clifton, NJ). 2022 Jan;2410:609–626.

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2022

Volume

2410

Start / End Page

609 / 626

Related Subject Headings

  • Vaccines
  • Phenotype
  • Neoplasms
  • Humans
  • Developmental Biology
  • Dendritic Cells
  • Cross-Priming
  • Cell Differentiation
  • Cancer Vaccines
  • 3404 Medicinal and biomolecular chemistry