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Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes.

Publication ,  Journal Article
Kaufman, RE; Kretschmer, PJ; Adams, JW; Coon, HC; Anderson, WF; Nienhuis, AW
Published in: Proc Natl Acad Sci U S A
July 1980

The human gamma-, delta-, and beta-globin genes are located within a 30-kilobase (kb) region of DNA, of which only 20% represents the globin genes. We have attempted to define the nature of flanking and intergenic sequences by isolating recombinants containing the human epsilon, both gamma-, or the 3' end of the beta-globin gene from a bacteriophage library of cloned human DNA. Comparison of these recombinants and a recombinant containing the delta- and beta-globin genes (H beta G1) has provided the following results. The epsilon-globin gene is located 14 kb 5' to the G gamma gene. DNA sequence homology between the region containing the two G gamma genes and the delta nd beta gene region is limited to only a few hundred nucleotides which include the globin coding sequences. Repetitive DNA sequences have been found in the region 3' to the beta-globin gene. Sequences located adjacent to the beta-globin gene are repeated in the globin gene region. A repetitive DNA sequence more than 3.2 kb long is repeated frequently in the human genome but is not repeated in the globin gene region in the clones examined.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 1980

Volume

77

Issue

7

Start / End Page

4229 / 4233

Location

United States

Related Subject Headings

  • Repetitive Sequences, Nucleic Acid
  • Humans
  • Globins
  • Genetic Linkage
  • Genes
  • Escherichia coli
  • DNA, Recombinant
  • DNA Restriction Enzymes
  • DNA
  • Cloning, Molecular
 

Citation

APA
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ICMJE
MLA
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Kaufman, R. E., Kretschmer, P. J., Adams, J. W., Coon, H. C., Anderson, W. F., & Nienhuis, A. W. (1980). Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes. Proc Natl Acad Sci U S A, 77(7), 4229–4233. https://doi.org/10.1073/pnas.77.7.4229
Kaufman, R. E., P. J. Kretschmer, J. W. Adams, H. C. Coon, W. F. Anderson, and A. W. Nienhuis. “Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes.Proc Natl Acad Sci U S A 77, no. 7 (July 1980): 4229–33. https://doi.org/10.1073/pnas.77.7.4229.
Kaufman RE, Kretschmer PJ, Adams JW, Coon HC, Anderson WF, Nienhuis AW. Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4229–33.
Kaufman, R. E., et al. “Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes.Proc Natl Acad Sci U S A, vol. 77, no. 7, July 1980, pp. 4229–33. Pubmed, doi:10.1073/pnas.77.7.4229.
Kaufman RE, Kretschmer PJ, Adams JW, Coon HC, Anderson WF, Nienhuis AW. Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4229–4233.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 1980

Volume

77

Issue

7

Start / End Page

4229 / 4233

Location

United States

Related Subject Headings

  • Repetitive Sequences, Nucleic Acid
  • Humans
  • Globins
  • Genetic Linkage
  • Genes
  • Escherichia coli
  • DNA, Recombinant
  • DNA Restriction Enzymes
  • DNA
  • Cloning, Molecular