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Vaccine with bacterium-like particles displaying HIV-1 gp120 trimer elicits specific mucosal responses and neutralizing antibodies in rhesus macaques.

Publication ,  Journal Article
Wang, H; Li, P; Zhang, M; Bi, J; He, Y; Li, F; Yu, R; Gao, F; Kong, W; Yu, B; Chen, L; Yu, X
Published in: Microb Biotechnol
July 2022

Preclinical studies have shown that the induction of secretory IgA (sIgA) in mucosa and neutralizing antibodies (NAbs) in sera is essential for designing vaccines that can effectively block the transmission of HIV-1. We previously showed that a vaccine consisting of bacterium-like particles (BLPs) displaying Protan-gp120AE-MTQ (PAM) could induce mucosal immune responses through intranasal (IN) immunization in mice and NAbs through intramuscular (IM) immunization in guinea pigs. Here, we evaluated the ability of this vaccine BLP-PAM to elicit HIV-1-specific mucosal and systemic immune responses through IN and IM immunization combination strategies in rhesus macaques. First, the morphology, antigenicity and epitope accessibility of the vaccine were analysed by transmission electron microscopy, bio-layer interferometry and ELISA. In BLP-PAM-immunized macaques, HIV-1-specific sIgA were rapidly induced through IN immunization in situ and distant mucosal sites, although the immune responses are relatively weak. Furthermore, the HIV-1-specific IgG and IgA antibody levels in mucosal secretions were enhanced and maintained, while production of serum NAbs against heterologous HIV-1 tier 1 and 2 pseudoviruses was elicited after IM boost. Additionally, situ mucosal responses and systemic T cell immune responses were improved by rAd2-gp120AE boost immunization via the IN and IM routes. These results suggested that BLP-based delivery in combination with the IN and IM immunization approach represents a potential vaccine strategy against HIV-1.

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Published In

Microb Biotechnol

DOI

EISSN

1751-7915

Publication Date

July 2022

Volume

15

Issue

7

Start / End Page

2022 / 2039

Location

United States

Related Subject Headings

  • Mucous Membrane
  • Mice
  • Macaca mulatta
  • Immunoglobulin G
  • Immunoglobulin A, Secretory
  • HIV-1
  • HIV Antibodies
  • Guinea Pigs
  • Antibodies, Neutralizing
  • Animals
 

Citation

APA
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MLA
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Wang, H., Li, P., Zhang, M., Bi, J., He, Y., Li, F., … Yu, X. (2022). Vaccine with bacterium-like particles displaying HIV-1 gp120 trimer elicits specific mucosal responses and neutralizing antibodies in rhesus macaques. Microb Biotechnol, 15(7), 2022–2039. https://doi.org/10.1111/1751-7915.14022
Wang, Huaiyu, Pingchao Li, Mo Zhang, Jinpeng Bi, Yizi He, Fangshen Li, Rongzhen Yu, et al. “Vaccine with bacterium-like particles displaying HIV-1 gp120 trimer elicits specific mucosal responses and neutralizing antibodies in rhesus macaques.Microb Biotechnol 15, no. 7 (July 2022): 2022–39. https://doi.org/10.1111/1751-7915.14022.
Wang, Huaiyu, et al. “Vaccine with bacterium-like particles displaying HIV-1 gp120 trimer elicits specific mucosal responses and neutralizing antibodies in rhesus macaques.Microb Biotechnol, vol. 15, no. 7, July 2022, pp. 2022–39. Pubmed, doi:10.1111/1751-7915.14022.
Wang H, Li P, Zhang M, Bi J, He Y, Li F, Yu R, Gao F, Kong W, Yu B, Chen L, Yu X. Vaccine with bacterium-like particles displaying HIV-1 gp120 trimer elicits specific mucosal responses and neutralizing antibodies in rhesus macaques. Microb Biotechnol. 2022 Jul;15(7):2022–2039.
Journal cover image

Published In

Microb Biotechnol

DOI

EISSN

1751-7915

Publication Date

July 2022

Volume

15

Issue

7

Start / End Page

2022 / 2039

Location

United States

Related Subject Headings

  • Mucous Membrane
  • Mice
  • Macaca mulatta
  • Immunoglobulin G
  • Immunoglobulin A, Secretory
  • HIV-1
  • HIV Antibodies
  • Guinea Pigs
  • Antibodies, Neutralizing
  • Animals