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Principles for the design of multicellular engineered living systems.

Publication ,  Journal Article
Aydin, O; Passaro, AP; Raman, R; Spellicy, SE; Weinberg, RP; Kamm, RD; Sample, M; Truskey, GA; Zartman, J; Dar, RD; Palacios, S; Wang, J ...
Published in: APL bioengineering
March 2022

Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell-cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the "black box" of living cells.

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Published In

APL bioengineering

DOI

EISSN

2473-2877

ISSN

2473-2877

Publication Date

March 2022

Volume

6

Issue

1

Start / End Page

010903

Related Subject Headings

  • 4003 Biomedical engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Aydin, O., Passaro, A. P., Raman, R., Spellicy, S. E., Weinberg, R. P., Kamm, R. D., … Weiss, R. (2022). Principles for the design of multicellular engineered living systems. APL Bioengineering, 6(1), 010903. https://doi.org/10.1063/5.0076635
Aydin, Onur, Austin P. Passaro, Ritu Raman, Samantha E. Spellicy, Robert P. Weinberg, Roger D. Kamm, Matthew Sample, et al. “Principles for the design of multicellular engineered living systems.APL Bioengineering 6, no. 1 (March 2022): 010903. https://doi.org/10.1063/5.0076635.
Aydin O, Passaro AP, Raman R, Spellicy SE, Weinberg RP, Kamm RD, et al. Principles for the design of multicellular engineered living systems. APL bioengineering. 2022 Mar;6(1):010903.
Aydin, Onur, et al. “Principles for the design of multicellular engineered living systems.APL Bioengineering, vol. 6, no. 1, Mar. 2022, p. 010903. Epmc, doi:10.1063/5.0076635.
Aydin O, Passaro AP, Raman R, Spellicy SE, Weinberg RP, Kamm RD, Sample M, Truskey GA, Zartman J, Dar RD, Palacios S, Wang J, Tordoff J, Montserrat N, Bashir R, Saif MTA, Weiss R. Principles for the design of multicellular engineered living systems. APL bioengineering. 2022 Mar;6(1):010903.

Published In

APL bioengineering

DOI

EISSN

2473-2877

ISSN

2473-2877

Publication Date

March 2022

Volume

6

Issue

1

Start / End Page

010903

Related Subject Headings

  • 4003 Biomedical engineering