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Growth of mesenchymal stem cells on electrospun type I collagen nanofibers.

Publication ,  Journal Article
Shih, Y-RV; Chen, C-N; Tsai, S-W; Wang, YJ; Lee, OK
Published in: Stem Cells
November 2006

We reconstituted type I collagen nanofibers prepared by electrospin technology and examined the morphology, growth, adhesion, cell motility, and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) on three nano-sized diameters (50-200, 200-500, and 500-1,000 nm). Results from scanning electron microscopy showed that cells on the nanofibers had a more polygonal and flattened cell morphology. MTS (3-[4,5-dimethythiazol-2-yl]-5-[3-carboxy-methoxyphenyl]-2-[4-sul-fophenyl]-2H-tetrazolium compound) assay demonstrated that the MSCs grown on 500-1,000-nm nanofibers had significantly higher cell viability than the tissue culture polystyrene control. A decreased amount of focal adhesion formation was apparent in which quantifiable staining area of the cytoplasmic protein vinculin for the 200-500-nm nanofibers was 39% less compared with control, whereas the area of quantifiable vinculin staining was 45% less for both the 200-500-nm and 500-1,000-nm nanofibers. The distances of cell migration were quantified on green fluorescent protein-nucleofected cells and was 56.7%, 37.3%, and 46.3% for 50-200, 200-500, and 500-1,000 nm, respectively, compared with those on the control. Alkaline phosphatase activity demonstrated no differences after 12 days of osteogenic differentiation, and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed comparable osteogenic gene expression of osteocalcin, osteonectin, and ostepontin between cells differentiated on polystyrene and nanofiber surfaces. Moreover, single-cell RT-PCR of type I collagen gene expression demonstrated higher expression on cells seeded on the nanofibers. Therefore, type I collagen nanofibers support the growth of MSCs without compromising their osteogenic differentiation capability and can be used as a scaffold for bone tissue engineering to facilitate intramembranous bone formation. Further efforts are necessary to enhance their biomimetic properties.

Duke Scholars

Published In

Stem Cells

DOI

ISSN

1066-5099

Publication Date

November 2006

Volume

24

Issue

11

Start / End Page

2391 / 2397

Location

England

Related Subject Headings

  • Tissue Engineering
  • RNA, Messenger
  • Particle Size
  • Osteopontin
  • Osteonectin
  • Osteogenesis
  • Osteocalcin
  • Osteoblasts
  • Nanostructures
  • Mesenchymal Stem Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shih, Y.-R., Chen, C.-N., Tsai, S.-W., Wang, Y. J., & Lee, O. K. (2006). Growth of mesenchymal stem cells on electrospun type I collagen nanofibers. Stem Cells, 24(11), 2391–2397. https://doi.org/10.1634/stemcells.2006-0253

Published In

Stem Cells

DOI

ISSN

1066-5099

Publication Date

November 2006

Volume

24

Issue

11

Start / End Page

2391 / 2397

Location

England

Related Subject Headings

  • Tissue Engineering
  • RNA, Messenger
  • Particle Size
  • Osteopontin
  • Osteonectin
  • Osteogenesis
  • Osteocalcin
  • Osteoblasts
  • Nanostructures
  • Mesenchymal Stem Cells