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Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT).

Publication ,  Journal Article
Li, M; Beaumont, N; Ma, C; Rojas, J; Vu, T; Harlacher, M; O'Connell, G; Gessner, RC; Kilian, H; Kasatkina, L; Chen, Y; Huang, Q; Shen, X ...
Published in: IEEE Trans Med Imaging
October 2022

Non-invasive small-animal imaging technologies, such as optical imaging, magnetic resonance imaging and x -ray computed tomography, have enabled researchers to study normal biological phenomena or disease progression in their native conditions. However, existing small-animal imaging technologies often lack either the penetration capability for interrogating deep tissues (e.g., optical microscopy), or the functional and molecular sensitivity for tracking specific activities (e.g., magnetic resonance imaging). To achieve functional and molecular imaging in deep tissues, we have developed an integrated photoacoustic, ultrasound and acoustic angiographic tomography (PAUSAT) system by seamlessly combining light and ultrasound. PAUSAT can perform three imaging modes simultaneously with complementary contrast: high-frequency B-mode ultrasound imaging of tissue morphology, microbubble-enabled acoustic angiography of tissue vasculature, and multi-spectral photoacoustic imaging of molecular probes. PAUSAT can provide three-dimensional (3D) multi-contrast images that are co-registered, with high spatial resolutions at large depths. Using PAUSAT, we performed proof-of-concept in vivo experiments on various small animal models: monitoring longitudinal development of placenta and embryo during mouse pregnancy, tracking biodistribution and metabolism of near-infrared organic dye on the whole-body scale, and detecting breast tumor expressing genetically-encoded photoswitchable phytochromes. These results have collectively demonstrated that PAUSAT has broad applicability in biomedical research, providing comprehensive structural, functional, and molecular imaging of small animal models.

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Published In

IEEE Trans Med Imaging

DOI

EISSN

1558-254X

Publication Date

October 2022

Volume

41

Issue

10

Start / End Page

2704 / 2714

Location

United States

Related Subject Headings

  • Ultrasonography
  • Tomography
  • Tissue Distribution
  • Photoacoustic Techniques
  • Nuclear Medicine & Medical Imaging
  • Molecular Probes
  • Molecular Imaging
  • Mice
  • Imaging, Three-Dimensional
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Li, M., Beaumont, N., Ma, C., Rojas, J., Vu, T., Harlacher, M., … Yao, J. (2022). Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT). IEEE Trans Med Imaging, 41(10), 2704–2714. https://doi.org/10.1109/TMI.2022.3168859
Li, Mucong, Nathan Beaumont, Chenshuo Ma, Juan Rojas, Tri Vu, Max Harlacher, Graeme O’Connell, et al. “Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT).IEEE Trans Med Imaging 41, no. 10 (October 2022): 2704–14. https://doi.org/10.1109/TMI.2022.3168859.
Li M, Beaumont N, Ma C, Rojas J, Vu T, Harlacher M, et al. Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT). IEEE Trans Med Imaging. 2022 Oct;41(10):2704–14.
Li, Mucong, et al. “Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT).IEEE Trans Med Imaging, vol. 41, no. 10, Oct. 2022, pp. 2704–14. Pubmed, doi:10.1109/TMI.2022.3168859.
Li M, Beaumont N, Ma C, Rojas J, Vu T, Harlacher M, O’Connell G, Gessner RC, Kilian H, Kasatkina L, Chen Y, Huang Q, Shen X, Lovell JF, Verkhusha VV, Czernuszewicz T, Yao J. Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT). IEEE Trans Med Imaging. 2022 Oct;41(10):2704–2714.

Published In

IEEE Trans Med Imaging

DOI

EISSN

1558-254X

Publication Date

October 2022

Volume

41

Issue

10

Start / End Page

2704 / 2714

Location

United States

Related Subject Headings

  • Ultrasonography
  • Tomography
  • Tissue Distribution
  • Photoacoustic Techniques
  • Nuclear Medicine & Medical Imaging
  • Molecular Probes
  • Molecular Imaging
  • Mice
  • Imaging, Three-Dimensional
  • Animals