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Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.

Publication ,  Journal Article
Garde, A; Sherwood, DR
Published in: STAR protocols
June 2022

Measuring ATP levels within the cytosol of living cells in animals is important to understand how cellular activities are energetically supported, but is challenging because of tissue complexity and ATP sensor limitations. In this protocol, we describe how to quantify ATP levels using PercevalHR in C. elegans larvae during anchor cell invasion. PercevalHR is a fluorescent biosensor that reports the cytoplasmic ATP:ADP ratio. The protocol can be adapted to analyze the ATP:ADP ratios within other cell types in C. elegans. For complete details on the use and execution of this protocol, please refer to Garde et al. (2022).

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Published In

STAR protocols

DOI

EISSN

2666-1667

ISSN

2666-1667

Publication Date

June 2022

Volume

3

Issue

2

Start / End Page

101429

Related Subject Headings

  • Larva
  • Cytosol
  • Caenorhabditis elegans
  • Animals
  • Adenosine Triphosphate
  • Adenosine Diphosphate
 

Citation

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Garde, A., & Sherwood, D. R. (2022). Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR. STAR Protocols, 3(2), 101429. https://doi.org/10.1016/j.xpro.2022.101429
Garde, Aastha, and David R. Sherwood. “Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.STAR Protocols 3, no. 2 (June 2022): 101429. https://doi.org/10.1016/j.xpro.2022.101429.
Garde A, Sherwood DR. Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR. STAR protocols. 2022 Jun;3(2):101429.
Garde, Aastha, and David R. Sherwood. “Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.STAR Protocols, vol. 3, no. 2, June 2022, p. 101429. Epmc, doi:10.1016/j.xpro.2022.101429.
Garde A, Sherwood DR. Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR. STAR protocols. 2022 Jun;3(2):101429.
Journal cover image

Published In

STAR protocols

DOI

EISSN

2666-1667

ISSN

2666-1667

Publication Date

June 2022

Volume

3

Issue

2

Start / End Page

101429

Related Subject Headings

  • Larva
  • Cytosol
  • Caenorhabditis elegans
  • Animals
  • Adenosine Triphosphate
  • Adenosine Diphosphate