Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells.

Publication ,  Journal Article
Schrott, R; Greeson, KW; King, D; Symosko Crow, KM; Easley, CA; Murphy, SK
Published in: Syst Biol Reprod Med
2022

Cannabis use in the United States is increasing, with highest consumption among men at their peak reproductive years. We previously demonstrated widespread changes in sperm DNA methylation with cannabis exposure in humans and rats, including genes important in neurodevelopment. Here, we use an in vitro human spermatogenesis model to recapitulate chronic cannabis use and assess DNA methylation at imprinted and autism spectrum disorder (ASD) candidate genes in spermatogonial stem cell (SSC)- and spermatid-like cells. Methylation at maternally imprinted genes SGCE and GRB10 was significantly altered in SSC- and spermatid-like cells, respectively, while PEG3 was significantly differentially methylated in spermatid-like cells. Two of ten randomly selected ASD candidate genes, HCN1 and NR4A2, had significantly altered methylation with cannabis exposure in SSC-like cells. These results support our findings in human cohorts and provide a new tool with which to gain mechanistic insights into the association between paternal cannabis use and risk of ASD in offspring.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Syst Biol Reprod Med

DOI

EISSN

1939-6376

Publication Date

2022

Volume

68

Issue

5-6

Start / End Page

357 / 369

Location

England

Related Subject Headings

  • Seeds
  • Rats
  • Obstetrics & Reproductive Medicine
  • Male
  • Humans
  • Genomic Imprinting
  • DNA Methylation
  • Cannabis
  • Autistic Disorder
  • Autism Spectrum Disorder
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Schrott, R., Greeson, K. W., King, D., Symosko Crow, K. M., Easley, C. A., & Murphy, S. K. (2022). Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells. Syst Biol Reprod Med, 68(5–6), 357–369. https://doi.org/10.1080/19396368.2022.2073292
Schrott, Rose, Katherine W. Greeson, Dillon King, Krista M. Symosko Crow, Charles A. Easley, and Susan K. Murphy. “Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells.Syst Biol Reprod Med 68, no. 5–6 (2022): 357–69. https://doi.org/10.1080/19396368.2022.2073292.
Schrott R, Greeson KW, King D, Symosko Crow KM, Easley CA, Murphy SK. Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells. Syst Biol Reprod Med. 2022;68(5–6):357–69.
Schrott, Rose, et al. “Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells.Syst Biol Reprod Med, vol. 68, no. 5–6, 2022, pp. 357–69. Pubmed, doi:10.1080/19396368.2022.2073292.
Schrott R, Greeson KW, King D, Symosko Crow KM, Easley CA, Murphy SK. Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells. Syst Biol Reprod Med. 2022;68(5–6):357–369.

Published In

Syst Biol Reprod Med

DOI

EISSN

1939-6376

Publication Date

2022

Volume

68

Issue

5-6

Start / End Page

357 / 369

Location

England

Related Subject Headings

  • Seeds
  • Rats
  • Obstetrics & Reproductive Medicine
  • Male
  • Humans
  • Genomic Imprinting
  • DNA Methylation
  • Cannabis
  • Autistic Disorder
  • Autism Spectrum Disorder