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Intelligent nanoscope for rapid nanomaterial identification and classification.

Publication ,  Journal Article
Jin, G; Hong, S; Rich, J; Xia, J; Kim, K; You, L; Zhao, C; Huang, TJ
Published in: Lab on a chip
August 2022

Machine learning image recognition and classification of particles and materials is a rapidly expanding field. However, nanomaterial identification and classification are dependent on the image resolution, the image field of view, and the processing time. Optical microscopes are one of the most widely utilized technologies in laboratories across the world, due to their nondestructive abilities to identify and classify critical micro-sized objects and processes, but identifying and classifying critical nano-sized objects and processes with a conventional microscope are outside of its capabilities, due to the diffraction limit of the optics and small field of view. To overcome these challenges of nanomaterial identification and classification, we developed an intelligent nanoscope that combines machine learning and microsphere array-based imaging to: (1) surpass the diffraction limit of the microscope objective with microsphere imaging to provide high-resolution images; (2) provide large field-of-view imaging without the sacrifice of resolution by utilizing a microsphere array; and (3) rapidly classify nanomaterials using a deep convolution neural network. The intelligent nanoscope delivers more than 46 magnified images from a single image frame so that we collected more than 1000 images within 2 seconds. Moreover, the intelligent nanoscope achieves a 95% nanomaterial classification accuracy using 1000 images of training sets, which is 45% more accurate than without the microsphere array. The intelligent nanoscope also achieves a 92% bacteria classification accuracy using 50 000 images of training sets, which is 35% more accurate than without the microsphere array. This platform accomplished rapid, accurate detection and classification of nanomaterials with miniscule size differences. The capabilities of this device wield the potential to further detect and classify smaller biological nanomaterial, such as viruses or extracellular vesicles.

Duke Scholars

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Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

August 2022

Volume

22

Issue

16

Start / End Page

2978 / 2985

Related Subject Headings

  • Neural Networks, Computer
  • Nanostructures
  • Microscopy
  • Machine Learning
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Jin, G., Hong, S., Rich, J., Xia, J., Kim, K., You, L., … Huang, T. J. (2022). Intelligent nanoscope for rapid nanomaterial identification and classification. Lab on a Chip, 22(16), 2978–2985. https://doi.org/10.1039/d2lc00206j
Jin, Geonsoo, Seongwoo Hong, Joseph Rich, Jianping Xia, Kyeri Kim, Lingchong You, Chenglong Zhao, and Tony Jun Huang. “Intelligent nanoscope for rapid nanomaterial identification and classification.Lab on a Chip 22, no. 16 (August 2022): 2978–85. https://doi.org/10.1039/d2lc00206j.
Jin G, Hong S, Rich J, Xia J, Kim K, You L, et al. Intelligent nanoscope for rapid nanomaterial identification and classification. Lab on a chip. 2022 Aug;22(16):2978–85.
Jin, Geonsoo, et al. “Intelligent nanoscope for rapid nanomaterial identification and classification.Lab on a Chip, vol. 22, no. 16, Aug. 2022, pp. 2978–85. Epmc, doi:10.1039/d2lc00206j.
Jin G, Hong S, Rich J, Xia J, Kim K, You L, Zhao C, Huang TJ. Intelligent nanoscope for rapid nanomaterial identification and classification. Lab on a chip. 2022 Aug;22(16):2978–2985.
Journal cover image

Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

August 2022

Volume

22

Issue

16

Start / End Page

2978 / 2985

Related Subject Headings

  • Neural Networks, Computer
  • Nanostructures
  • Microscopy
  • Machine Learning
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences