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FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction.

Publication ,  Journal Article
Corbin Goodman, LC; Erickson, HP
Published in: Microbiology (Reading)
June 2022

There has been recent debate as to the source of constriction force during cell division. FtsZ can generate a constriction force on tubular membranes in vitro, suggesting it may generate the constriction force in vivo. However, another study showed that mutants of FtsZ did not affect the rate of constriction, whereas mutants of the PG assembly did, suggesting that PG assembly may push the constriction from the outside. Supporting this model, two groups found that cells that have initiated constriction can complete septation while the Z ring is poisoned with the FtsZ targeting antibiotic PC190723. PC19 arrests treadmilling but leaves FtsZ in place. We sought to determine if a fully assembled Z ring is necessary during constriction. To do this, we used a temperature-sensitive FtsZ mutant, FtsZ84. FtsZ84 supports cell division at 30 °C, but it disassembles from the Z ring within 1 min upon a temperature jump to 42 °C. Following the temperature jump we found that cells in early constriction stop constricting. Cells that had progressed to the later stage of division finished constriction without a Z ring. These results show that in Escherichia coli, an assembled Z ring is essential for constriction except in the final stage, contradicting the simplest interpretation of previous studies using PC19.

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Published In

Microbiology (Reading)

DOI

EISSN

1465-2080

Publication Date

June 2022

Volume

168

Issue

6

Location

England

Related Subject Headings

  • Microbiology
  • Escherichia coli Proteins
  • Escherichia coli
  • Cytoskeletal Proteins
  • Constriction
  • Cell Division
  • Bacterial Proteins
 

Citation

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Corbin Goodman, L. C., & Erickson, H. P. (2022). FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction. Microbiology (Reading), 168(6). https://doi.org/10.1099/mic.0.001194
Corbin Goodman, Lauren C., and Harold P. Erickson. “FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction.Microbiology (Reading) 168, no. 6 (June 2022). https://doi.org/10.1099/mic.0.001194.
Corbin Goodman LC, Erickson HP. FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction. Microbiology (Reading). 2022 Jun;168(6).
Corbin Goodman, Lauren C., and Harold P. Erickson. “FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction.Microbiology (Reading), vol. 168, no. 6, June 2022. Pubmed, doi:10.1099/mic.0.001194.
Corbin Goodman LC, Erickson HP. FtsZ at mid-cell is essential in Escherichia coli until the late stage of constriction. Microbiology (Reading). 2022 Jun;168(6).

Published In

Microbiology (Reading)

DOI

EISSN

1465-2080

Publication Date

June 2022

Volume

168

Issue

6

Location

England

Related Subject Headings

  • Microbiology
  • Escherichia coli Proteins
  • Escherichia coli
  • Cytoskeletal Proteins
  • Constriction
  • Cell Division
  • Bacterial Proteins