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Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds.

Publication ,  Journal Article
Wilson, KL; Pérez, SCL; Naffaa, MM; Kelly, SH; Segura, T
Published in: Advanced materials (Deerfield Beach, Fla.)
August 2022

Microporous annealed particle (MAP) scaffolds are generated from assembled hydrogel microparticles (microgels). It has been previously demonstrated that MAP scaffold are porous, biocompatible, and recruit neural progenitor cells (NPCs) to the stroke cavity after injection into the stroke core. Here, the goal is to study NPC fate inside MAP scaffolds in vitro. To create plain microgels that can later be converted to contain different types of bioactivities, the inverse electron-demand Diels-Alder reaction between tetrazine and norbornene is utilized, which allows the post-modification of plain microgels stoichiometrically. As a result of adhesive peptide attachment, NPC spreading leads to contractile force generation which can be recorded by tracking microgel displacement. Alternatively, non-adhesive peptide integration results in neurosphere formation that grows within the void space of MAP scaffolds. Although the formed neurospheres do not impose a contractile force on the scaffolds, they are seen to continuously transverse the scaffolds. It is concluded that MAP scaffolds  can be engineered to either promote neurogenesis or enhance stemness depending on the chosen post-modifications of the microgels, which can be key in modulating their phenotypes in various applications in vivo.

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Published In

Advanced materials (Deerfield Beach, Fla.)

DOI

EISSN

1521-4095

ISSN

0935-9648

Publication Date

August 2022

Volume

34

Issue

33

Start / End Page

e2201921

Related Subject Headings

  • Tissue Scaffolds
  • Stroke
  • Neural Stem Cells
  • Nanoscience & Nanotechnology
  • Microgels
  • Hydrogels
  • Humans
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
 

Citation

APA
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ICMJE
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Wilson, K. L., Pérez, S. C. L., Naffaa, M. M., Kelly, S. H., & Segura, T. (2022). Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds. Advanced Materials (Deerfield Beach, Fla.), 34(33), e2201921. https://doi.org/10.1002/adma.202201921
Wilson, Katrina L., Sasha Cai Lesher Pérez, Moawiah M. Naffaa, Sean H. Kelly, and Tatiana Segura. “Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds.Advanced Materials (Deerfield Beach, Fla.) 34, no. 33 (August 2022): e2201921. https://doi.org/10.1002/adma.202201921.
Wilson KL, Pérez SCL, Naffaa MM, Kelly SH, Segura T. Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds. Advanced materials (Deerfield Beach, Fla). 2022 Aug;34(33):e2201921.
Wilson, Katrina L., et al. “Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds.Advanced Materials (Deerfield Beach, Fla.), vol. 34, no. 33, Aug. 2022, p. e2201921. Epmc, doi:10.1002/adma.202201921.
Wilson KL, Pérez SCL, Naffaa MM, Kelly SH, Segura T. Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds. Advanced materials (Deerfield Beach, Fla). 2022 Aug;34(33):e2201921.
Journal cover image

Published In

Advanced materials (Deerfield Beach, Fla.)

DOI

EISSN

1521-4095

ISSN

0935-9648

Publication Date

August 2022

Volume

34

Issue

33

Start / End Page

e2201921

Related Subject Headings

  • Tissue Scaffolds
  • Stroke
  • Neural Stem Cells
  • Nanoscience & Nanotechnology
  • Microgels
  • Hydrogels
  • Humans
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences