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Anti-LAMP-2 antibodies are not prevalent in patients with antineutrophil cytoplasmic autoantibody glomerulonephritis.

Publication ,  Journal Article
Roth, AJ; Brown, MC; Smith, RN; Badhwar, AK; Parente, O; Chung, HC; Bunch, DO; McGregor, JG; Hogan, SL; Hu, Y; Yang, J-J; Berg, EA; Niles, J ...
Published in: J Am Soc Nephrol
March 2012

Lysosomal membrane protein 2 (LAMP-2) is a target of antineutrophil cytoplasmic autoantibodies (ANCA) in addition to the more commonly known targets proteinase 3 and myeloperoxidase. The prevalence of anti-LAMP-2 antibodies and their relationship to disease in ANCA glomerulonephritis are not well described. We measured anti-LAMP-2 reactivity in 680 sera samples (two academic centers) from patients with ANCA glomerulonephritis (n=329); those with ANCA-negative glomerulonephritis (n=104); those with fimbriated, gram-negative Escherichia coli urinary tract infection (n=104); disease controls (n=19); and healthy volunteers (n=124). With levels in healthy controls used to define a reference range, anti-LAMP-2 reactivity was present in 21% of ANCA sera from two of the centers; reactivity was present in 16% of the control group with urinary tract infection. Western blotting and immunofluorescence microscopy did not verify positivity. Titers of anti-myeloperoxidase and anti-proteinase 3 antibodies were 1500-fold and 10,000-fold higher than anti-LAMP-2 titers, respectively. There was no correlation between anti-LAMP-2 antibodies and disease activity. Furthermore, Wistar Kyoto rats injected with anti-LAMP-2 antibodies did not develop glomerulonephritis. In conclusion, antibodies that react with LAMP-2 may exist at very low titers in a minority of patients with ANCA disease. These data do not support a mechanistic relationship between anti-LAMP-2 antibodies and ANCA glomerulonephritis.

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Published In

J Am Soc Nephrol

DOI

EISSN

1533-3450

Publication Date

March 2012

Volume

23

Issue

3

Start / End Page

545 / 555

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Urinary Tract Infections
  • Sensitivity and Specificity
  • Rats, Inbred WKY
  • Rats
  • Prevalence
  • Peroxidase
  • Myeloblastin
  • Middle Aged
  • Male
 

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Roth, A. J., Brown, M. C., Smith, R. N., Badhwar, A. K., Parente, O., Chung, H. C., … Falk, R. J. (2012). Anti-LAMP-2 antibodies are not prevalent in patients with antineutrophil cytoplasmic autoantibody glomerulonephritis. J Am Soc Nephrol, 23(3), 545–555. https://doi.org/10.1681/ASN.2011030273
Roth, Aleeza J., Michael C. Brown, Rex Neal Smith, Anshul K. Badhwar, Oscar Parente, Hyun chul Chung, Donna O’Dell Bunch, et al. “Anti-LAMP-2 antibodies are not prevalent in patients with antineutrophil cytoplasmic autoantibody glomerulonephritis.J Am Soc Nephrol 23, no. 3 (March 2012): 545–55. https://doi.org/10.1681/ASN.2011030273.
Roth AJ, Brown MC, Smith RN, Badhwar AK, Parente O, Chung HC, et al. Anti-LAMP-2 antibodies are not prevalent in patients with antineutrophil cytoplasmic autoantibody glomerulonephritis. J Am Soc Nephrol. 2012 Mar;23(3):545–55.
Roth, Aleeza J., et al. “Anti-LAMP-2 antibodies are not prevalent in patients with antineutrophil cytoplasmic autoantibody glomerulonephritis.J Am Soc Nephrol, vol. 23, no. 3, Mar. 2012, pp. 545–55. Pubmed, doi:10.1681/ASN.2011030273.
Roth AJ, Brown MC, Smith RN, Badhwar AK, Parente O, Chung HC, Bunch DO, McGregor JG, Hogan SL, Hu Y, Yang J-J, Berg EA, Niles J, Jennette JC, Preston GA, Falk RJ. Anti-LAMP-2 antibodies are not prevalent in patients with antineutrophil cytoplasmic autoantibody glomerulonephritis. J Am Soc Nephrol. 2012 Mar;23(3):545–555.

Published In

J Am Soc Nephrol

DOI

EISSN

1533-3450

Publication Date

March 2012

Volume

23

Issue

3

Start / End Page

545 / 555

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Urinary Tract Infections
  • Sensitivity and Specificity
  • Rats, Inbred WKY
  • Rats
  • Prevalence
  • Peroxidase
  • Myeloblastin
  • Middle Aged
  • Male