Scholars@Duke
Journal cover image

Regulation of cold-induced thermogenesis by the RNA binding protein FAM195A.

Publication ,  Journal Article
Cannavino, J; Shao, M; An, YA; Bezprozvannaya, S; Chen, S; Kim, J; Xu, L; McAnally, JR; Scherer, PE; Liu, N; Gupta, RK; Bassel-Duby, R; Olson, EN
Published in: Proc Natl Acad Sci U S A
June 8, 2021
Published version (DOI) Link to item

Homeothermic vertebrates produce heat in cold environments through thermogenesis, in which brown adipose tissue (BAT) increases mitochondrial oxidation along with uncoupling of the electron transport chain and activation of uncoupling protein 1 (UCP1). Although the transcription factors regulating the expression of UCP1 and nutrient oxidation genes have been extensively studied, only a few other proteins essential for BAT function have been identified. We describe the discovery of FAM195A, a BAT-enriched RNA binding protein, which is required for cold-dependent thermogenesis in mice. FAM195A knockout (KO) mice display whitening of BAT and an inability to thermoregulate. In BAT of FAM195A KO mice, enzymes involved in branched-chain amino acid (BCAA) metabolism are down-regulated, impairing their response to cold. Knockdown of FAM195A in brown adipocytes in vitro also impairs expression of leucine oxidation enzymes, revealing FAM195A to be a regulator of BCAA metabolism and a potential target for metabolic disorders.

Duke Scholars

Jessica Cannavino
Author Jessica Cannavino Medicine, Endocrinology, Metabolism, and Nutrition
Rana K. Gupta
Author Rana K. Gupta Medicine, Endocrinology, Metabolism, and Nutrition
Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.2104650118

EISSN

1091-6490

Publication Date

June 8, 2021

Volume

118

Issue

23

Location

United States

Related Subject Headings

  • Thermogenesis
  • Mice, Knockout
  • Mice
  • Intracellular Signaling Peptides and Proteins
  • Cold Temperature
  • Cell Line, Transformed
  • Animals
  • Amino Acids, Branched-Chain
  • Adipose Tissue, Brown
  • Adipocytes, Brown
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cannavino, J., Shao, M., An, Y. A., Bezprozvannaya, S., Chen, S., Kim, J., … Olson, E. N. (2021). Regulation of cold-induced thermogenesis by the RNA binding protein FAM195A. Proc Natl Acad Sci U S A, 118(23). https://doi.org/10.1073/pnas.2104650118
Cannavino, Jessica, Mengle Shao, Yu A. An, Svetlana Bezprozvannaya, Shiuhwei Chen, Jiwoong Kim, Lin Xu, et al. “Regulation of cold-induced thermogenesis by the RNA binding protein FAM195A.” Proc Natl Acad Sci U S A 118, no. 23 (June 8, 2021). https://doi.org/10.1073/pnas.2104650118.
Cannavino J, Shao M, An YA, Bezprozvannaya S, Chen S, Kim J, et al. Regulation of cold-induced thermogenesis by the RNA binding protein FAM195A. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23).
Cannavino, Jessica, et al. “Regulation of cold-induced thermogenesis by the RNA binding protein FAM195A.” Proc Natl Acad Sci U S A, vol. 118, no. 23, June 2021. Pubmed, doi:10.1073/pnas.2104650118.
Cannavino J, Shao M, An YA, Bezprozvannaya S, Chen S, Kim J, Xu L, McAnally JR, Scherer PE, Liu N, Gupta RK, Bassel-Duby R, Olson EN. Regulation of cold-induced thermogenesis by the RNA binding protein FAM195A. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23).
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.2104650118

EISSN

1091-6490

Publication Date

June 8, 2021

Volume

118

Issue

23

Location

United States

Related Subject Headings

  • Thermogenesis
  • Mice, Knockout
  • Mice
  • Intracellular Signaling Peptides and Proteins
  • Cold Temperature
  • Cell Line, Transformed
  • Animals
  • Amino Acids, Branched-Chain
  • Adipose Tissue, Brown
  • Adipocytes, Brown