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Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning.

Publication ,  Journal Article
Neinast, MD; Frank, AP; Zechner, JF; Li, Q; Vishvanath, L; Palmer, BF; Aguirre, V; Gupta, RK; Clegg, DJ
Published in: Mol Metab
May 2015

OBJECTIVE: Roux-en-Y gastric bypass (RYGB) is an effective method of weight loss and remediation of type-2 diabetes; however, the mechanisms leading to these improvements are unclear. Additionally, adipocytes within white adipose tissue (WAT) depots can manifest characteristics of brown adipocytes. These 'BRITE/beige' adipocytes express uncoupling protein 1 (UCP1) and are associated with improvements in glucose homeostasis and protection from obesity. Interestingly, atrial and B-type natriuretic peptides (NPs) promote BRITE/beige adipocyte enrichment of WAT depots, an effect known as "browning." Here, we investigate the effect of RYGB surgery on NP, NP receptors, and browning in the gonadal adipose tissues of female mice. We propose that such changes may lead to improvements in metabolic homeostasis commonly observed following RYGB. METHODS: Wild type, female, C57/Bl6 mice were fed a 60% fat diet ad libitum for six months. Mice were divided into three groups: Sham operated (SO), Roux-en-Y gastric bypass (RYGB), and Weight matched, sham operated (WM-SO). Mice were sacrificed six weeks following surgery and evaluated for differences in body weight, glucose homeostasis, adipocyte morphology, and adipose tissue gene expression. RESULTS: RYGB and calorie restriction induced similar weight loss and improved glucose metabolism without decreasing food intake. β3-adrenergic receptor expression increased in gonadal adipose tissue, in addition to Nppb (BNP), and NP receptors, Npr1, and Npr2. The ratio of Npr1:Npr3 and Npr2:Npr3 increased in RYGB, but not WM-SO groups. Ucp1 protein and mRNA, as well as additional markers of BRITE/beige adipose tissue and lipolytic genes increased in RYGB mice to a greater extent than calorie-restricted mice. CONCLUSIONS: Upregulation of Nppb, Npr1, Npr2, and β3-adrenergic receptors in gonadal adipose tissue following RYGB was associated with increased markers of browning. This browning of gonadal adipose tissue may underpin the positive effect of RYGB on metabolic parameters and may in part be mediated through upregulation of natriuretic peptides.

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Published In

Mol Metab

DOI

ISSN

2212-8778

Publication Date

May 2015

Volume

4

Issue

5

Start / End Page

427 / 436

Location

Germany

Related Subject Headings

  • 3101 Biochemistry and cell biology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
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Neinast, M. D., Frank, A. P., Zechner, J. F., Li, Q., Vishvanath, L., Palmer, B. F., … Clegg, D. J. (2015). Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning. Mol Metab, 4(5), 427–436. https://doi.org/10.1016/j.molmet.2015.02.006
Neinast, Michael D., Aaron P. Frank, Juliet F. Zechner, Quanlin Li, Lavanya Vishvanath, Biff F. Palmer, Vincent Aguirre, Rana K. Gupta, and Deborah J. Clegg. “Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning.Mol Metab 4, no. 5 (May 2015): 427–36. https://doi.org/10.1016/j.molmet.2015.02.006.
Neinast MD, Frank AP, Zechner JF, Li Q, Vishvanath L, Palmer BF, et al. Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning. Mol Metab. 2015 May;4(5):427–36.
Neinast, Michael D., et al. “Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning.Mol Metab, vol. 4, no. 5, May 2015, pp. 427–36. Pubmed, doi:10.1016/j.molmet.2015.02.006.
Neinast MD, Frank AP, Zechner JF, Li Q, Vishvanath L, Palmer BF, Aguirre V, Gupta RK, Clegg DJ. Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning. Mol Metab. 2015 May;4(5):427–436.
Journal cover image

Published In

Mol Metab

DOI

ISSN

2212-8778

Publication Date

May 2015

Volume

4

Issue

5

Start / End Page

427 / 436

Location

Germany

Related Subject Headings

  • 3101 Biochemistry and cell biology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology